Transduction of porcine enteropathogenic Escherichia coli with a derivative of a Shiga toxin 2-encoding bacteriophage in a porcine ligated ileal loop system
| Autor: | Mohamed A. Dow, Anna Malik, Istvan Toth, Eric Oswald, Béla Nagy, Herbert Schmidt |
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| Přispěvatelé: | Inconnu, Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées |
| Jazyk: | angličtina |
| Rok vydání: | 2003 |
| Předmět: |
Swine
Virus Integration [SDV]Life Sciences [q-bio] Genetics and Molecular Biology medicine.disease_cause Coliphages Shiga Toxin 2 Applied Microbiology and Biotechnology Microbiology Bacteriophage 03 medical and health sciences chemistry.chemical_compound Shiga-like toxin Ileum Transduction Genetic In vivo Lysogenic cycle Escherichia coli medicine Animals Enteropathogenic Escherichia coli ComputingMilieux_MISCELLANEOUS 030304 developmental biology 0303 health sciences Ecology biology 030306 microbiology Shiga toxin biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Enterobacteriaceae chemistry biology.protein bacteria Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology Applied and Environmental Microbiology, American Society for Microbiology, 2003, 69 (12), pp.7242-7247. ⟨10.1128/AEM.69.12.7242-7247.2003⟩ |
| ISSN: | 0099-2240 1098-5336 |
| DOI: | 10.1128/AEM.69.12.7242-7247.2003⟩ |
| Popis: | In this study, we have investigated the ability of detoxified Shiga toxin (Stx)-converting bacteriophages Φ3538 (Δ stx 2 :: cat ) (H. Schmidt et al., Appl. Environ. Microbiol. 65:3855-3861, 1999) and H-19B::Tn 10d-bla (D. W. Acheson et al., Infect. Immun. 66:4496-4498, 1998) to lysogenize enteropathogenic Escherichia coli (EPEC) strains in vivo. We were able to transduce the porcine EPEC strain 1390 (O45) withΦ 3538 (Δ stx 2 :: cat ) in porcine ligated ileal loops but not the human EPEC prototype strain E2348/69 (O127). Neither strain 1390 nor strain E2348/69 was lysogenized under these in vivo conditions when E. coli K-12 containing H-19B::Tn 10d-bla was used as the stx1 phage donor. The repeated success in the in vivo transduction of an Stx2-encoding phage to a porcine EPEC strain in pig loops was in contrast to failures in the in vitro trials with these and other EPEC strains. These results indicate that in vivo conditions are more effective for transduction of Stx2-encoding phages than in vitro conditions. |
| Databáze: | OpenAIRE |
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