Doxycycline Inhibits IL-17-Stimulated MMP-9 Expression by Downregulating ERK1/2 Activation: Implications in Myogenic Differentiation
| Autor: | Aleksandra Jauković, Hristina Obradović, Drenka Trivanović, Diana Bugarski, Tamara Kukolj, Juan F. Santibanez, Gordana Jovčić, Jelena Krstic, Slavko Mojsilović, Ivana Okić Đorđević |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Article Subject MAP Kinase Signaling System Cellular differentiation Immunology Inflammation Biology Matrix metalloproteinase Muscle Development Cell Line Myoblasts 03 medical and health sciences Mice Internal medicine medicine lcsh:Pathology Myocyte Animals Muscle Skeletal Myogenesis Interleukin-17 Skeletal muscle Cell Differentiation Cell Biology Recombinant Proteins 3. Good health Cell biology 030104 developmental biology medicine.anatomical_structure Endocrinology Gene Expression Regulation Matrix Metalloproteinase 9 Doxycycline Interleukin 17 medicine.symptom C2C12 lcsh:RB1-214 Research Article |
| Zdroj: | Mediators of Inflammation Mediators of Inflammation, Vol 2016 (2016) |
| ISSN: | 1466-1861 |
| Popis: | Interleukin 17 (IL-17) is a cytokine with pleiotropic effects associated with several inflammatory diseases. Although elevated levels of IL-17 have been described in inflammatory myopathies, its role in muscle remodeling and regeneration is still unknown. Excessive extracellular matrix degradation in skeletal muscle is an important pathological consequence of many diseases involving muscle wasting. In this study, the role of IL-17 on the expression of matrix metalloproteinase- (MMP-) 9 in myoblast cells was investigated. The expression of MMP-9 after IL-17 treatment was analyzed in mouse myoblasts C2C12 cell line. The increase in MMP-9 production by IL-17 was concomitant with its capacity to inhibit myogenic differentiation of C2C12 cells. Doxycycline (Doxy) treatment protected the myogenic capacity of myoblasts from IL-17 inhibition and, moreover, increased myotubes hypertrophy. Doxy blocked the capacity of IL-17 to stimulate MMP-9 production by regulating IL-17-induced ERK1/2 MAPK activation. Our results imply that MMP-9 mediates IL-17’s capacity to inhibit myoblast differentiation during inflammatory diseases and indicate that Doxy can modulate myoblast response to inflammatory induction by IL-17. |
| Databáze: | OpenAIRE |
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