High-resolution synchrotron radiation-based phase tomography of the healthy and epileptic brain
| Autor: | Natalia Chicherova, Luigi Mariani, Hans Deyhle, Bert Müller, Philipp Janz, Carola A. Haas, Anna Khimchenko, Alexander Rack, Christos Bikis, Simone E. Hieber, Peter Thalmann, Georg Schulz, Jürgen Hench, Gabriel Schweighauser |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Physics Focus (geometry) business.industry Dentate gyrus Thalamus Hippocampus Ventricular system medicine.disease 03 medical and health sciences Epilepsy 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Optics Cortex (anatomy) medicine Tomography business 030217 neurology & neurosurgery Biomedical engineering |
| Zdroj: | DEVELOPMENTS IN X-RAY TOMOGRAPHY X |
| DOI: | 10.1117/12.2237816 |
| Popis: | Phase-contrast micro-tomography using synchrotron radiation has yielded superior soft tissue visualization down to the sub-cellular level. The isotropic spatial resolution down to about one micron is comparable to the one of histology. The methods, however, provide different physical quantities and are thus complementary, also allowing for the extension of histology into the third dimension. To prepare for cross-sectional animal studies on epilepsy, we have standardized the specimen’s preparation and scanning procedure for mouse brains, so that subsequent histology remains entirely unaffected and scanning of all samples (n = 28) is possible in a realistic time frame. For that, we have scanned five healthy and epileptic mouse brains at the ID19 beamline, ESRF, Grenoble, France, using grating- and propagation-based phase contrast micro-tomography. The resulting datasets clearly show the cortex, ventricular system, thalamus, hypothalamus, and hippocampus. Our focus is on the latter, having planned kainate-induced epilepsy experiments. The cell density and organization in the dentate gyrus and Ammon’s horn region were clearly visualized in control animals. This proof of principle was required to initiate experiment. The resulting three-dimensional data have been correlated to histology. The goal is a brain-wide quantification of cell death or structural reorganization associated with epilepsy as opposed to histology alone that represents small volumes of the total brain only. Thus, the proposed technique bears the potential to correlate the gold standard in analysis with independently obtained data sets. Such an achievement also fuels interest for other groups in neuroscience research to closely collaborate with experts in phase micro-tomography. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|