Growth/differentiation factor-15 and its role in peripheral nervous system lesion and regeneration
| Autor: | Andreas Schober, Frank Bosse, Petar Charalambous, Xiaolong Wang, Klaus Unsicker, H. W. Müller, Venissa Machado, Julia Krebbers |
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| Rok vydání: | 2015 |
| Předmět: |
Nervous system
Growth Differentiation Factor 15 Histology Nerve Crush Mice Transgenic Pathology and Forensic Medicine Lesion Transforming Growth Factor beta Ganglia Spinal medicine Animals Remyelination Axon biology Cell Biology Transforming growth factor beta Sciatic Nerve Axons Nerve Regeneration Cell biology Mice Inbred C57BL medicine.anatomical_structure nervous system Peripheral nervous system biology.protein GDF15 Sciatic nerve medicine.symptom Neuroscience |
| Zdroj: | Cell and Tissue Research. 362:317-330 |
| ISSN: | 1432-0878 0302-766X |
| Popis: | Growth/differentiation factor-15 (GDF-15) is a distant member of the transforming growth factor beta (TGF-β) superfamily. It is widely distributed in the nervous system, where it has been shown to play an important role in neuronal maintenance. The present study investigates the role of endogenous GDF-15 in sciatic nerve (SN) lesions using wild-type (WT) and GDF-15 knock-out (KO) mice. SN of 5-6-month-old mice were crushed or transected. Dorsal root ganglia (DRG) and nerve tissue were analyzed at different time points from 6 h to 9 weeks post-lesion. Both crush and transection induced GDF-15 mRNA and protein in the distal portion of the nerve, with a peak at day 7. DRG neuron death did not significantly differ between the genotypes; similarly, remyelination of regenerating axons was not affected by the genotype. Alternative macrophage activation and macrophage recruitment were more pronounced in the KO nerve. Protrusion speed of axons was similar in the two genotypes but WT axons showed better maturation, as indicated by larger caliber at 9 weeks. Furthermore, the regenerated WT nerve showed better performance in the electromyography test, indicating better functional recovery. We conclude that endogenous GDF-15 is beneficial for axon regeneration following SN crush. |
| Databáze: | OpenAIRE |
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