Application of Adeno-Associated Virus Vectors for Engineering SCF-Containing Extracellular Vesicles of Mesenchymal Stromal Cells
| Autor: | M. Yu. Men’shikov, E. S. Zubkova, A. T. Kopylov, I. B. Beloglazova, Evgeniy G. Evtushenko, E. V. Parfenova, K. V. Dergilev, E. I. Ratner, E.K. Shevchenko |
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| Rok vydání: | 2019 |
| Předmět: |
Proteomics
0301 basic medicine Transgene Adipose tissue Nanoparticle tracking analysis Stem cell factor medicine.disease_cause Mass Spectrometry General Biochemistry Genetics and Molecular Biology Extracellular Vesicles 03 medical and health sciences 0302 clinical medicine medicine Animals Adeno-associated virus Cells Cultured Stem Cell Factor Chemistry Mesenchymal stem cell Mesenchymal Stem Cells General Medicine Dependovirus Rats Cell biology Blot 030104 developmental biology Adipose Tissue 030217 neurology & neurosurgery Immunostaining |
| Zdroj: | Bulletin of Experimental Biology and Medicine. 166:527-534 |
| ISSN: | 1573-8221 0007-4888 |
| DOI: | 10.1007/s10517-019-04387-2 |
| Popis: | Mesenchymal stromal cells from rat adipose tissue were transduced with adeno-associated viral (AAV) vector encoding stem cell factor SCF that stimulates proliferation of cardiac c-kit+ cells and improved cardiac function and survival of animals after myocardial infarction. Extracellular vesicles isolated from the medium conditioned by mesenchymal stromal cells by ultracentrifugation were characterized by Western blotting, transmission electron microscopy, nanoparticle tracking analysis, immunostaining, and mass spectrometry analysis. Using proteomic analysis, we identified transgenic SCF in extracellular vesicles released by AAV-modified mesenchymal stromal cells and detected some proteins specific of extracellular vesicles secreted by transduced cells. Extracellular vesicles from AAV-transduced mesenchymal stromal cells could be used for delivery of transgenic proteins as they were readily endocytosed by both cardiosphere-derived cells and cardiac-progenitor cells. |
| Databáze: | OpenAIRE |
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