Production and optimization of xylooligosaccharides from corncob by Bacillus aerophilus KGJ2 xylanase and its antioxidant potential
| Autor: | V. Ponnusami, D. Gowdhaman |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
animal structures
DPPH ved/biology.organism_classification_rank.species Bacillus Corncob Zea mays Biochemistry chemistry.chemical_compound Bacterial Proteins Structural Biology Enzymatic hydrolysis Food science Molecular Biology Endo-1 4-beta Xylanases Plant Extracts Chemistry ved/biology technology industry and agriculture Free Radical Scavengers General Medicine Xylan Bacillus aerophilus Yield (chemistry) Sodium hypochlorite Xylanase Xylans |
| Zdroj: | International Journal of Biological Macromolecules. 79:595-600 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2015.05.046 |
| Popis: | The aim of the present study is to produce xylooligosaccharides (XOS) from corncob xylan. The xylan was extracted from corncob using methods like dilute acid, dilute alkali and sodium hypochlorite treatment. Corncob xylan extracted using alkali was characterized by FT-IR and TG-DSC. The extracted xylan was subjected to enzymatic hydrolysis using Bacillus aerophilus KGJ2 xylanase for XOS production. To increase the yield of XOS, the effects of various process parameters like substrate concentration, reaction time, and enzyme concentration on XOS production were investigated. XOS prepared was characterized by HPTLC. Anti oxidant potential of produced XOS was evaluated and the DPPH assay showed that XOS possessed concentration dependent free radical scavenging activity. |
| Databáze: | OpenAIRE |
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