Helicobacter pylori virulence dupA gene: risk factor or protective factor?
| Autor: | Ana Karoline Silva Oliveira, Mônica Santiago Barbosa, Lucas Luiz de Lima Silva, Amanda Ferreira Paes Landim Ramos, Aline Rodrigues Gama, Lucas Trevizani Rasmussem, José Daniel Gonçalves Vieira, Angel José Vieira Blanco, Lilian Carla Carneiro, Antonio Márcio Teodoro Cordeiro Silva |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
medicine.medical_specialty Clinical Microbiology - Research Paper Virulence Factors Chronic gastritis Virulence Microbiology Virulence factor Helicobacter Infections Medical microbiology Bacterial Proteins Risk Factors RNA Ribosomal 16S Media Technology medicine Humans Risk factor Dyspepsia Gene Phylogeny biology Helicobacter pylori Protective Factors medicine.disease biology.organism_classification Female Gastritis medicine.symptom Brazil |
| Zdroj: | Braz J Microbiol |
| Popis: | Helicobacter pylori is the etiological agent of chronic gastritis, peptic ulcer, and gastric cancer. The duodenal ulcer-promoting gene dupA, which is located in the plasticity region of the H. pylori genome, is homologous to the virB gene which encodes a type IV secretion protein in Agrobacterium tumefaciens. Studies have shown associations between H. pylori dupA-positive strains and gastroduodenal diseases. However, whether dupA acts as a risk factor or protective factor in these diseases remains unclear. Therefore, in this study, we aimed to verify the presence of the dupA gene in infectious H. pylori strains in the Brazilian mid-west and to investigate its association with the clinical outcomes of patients with dyspepsia. Additionally, the phylogenetic origin of the strains was determined. Gastric biopsies from 117 patients with dyspepsia were analyzed using histological and molecular techniques. The hpx gene (16S rRNA) was used to screen for H. pylori infection, and positive samples were then subjected to dupA gene detection and sequencing. The estimated prevalence of H. pylori infection was 64.1%, with the dupA gene being detected in a high proportion of infectious strains (70.7%). Furthermore, a risk analysis revealed that for women, a dupA-positive H. pylori infection increased the chance of developing gastritis by twofold. The partial dupA sequences from isolated infectious strains in this work are similar to those of strains isolated in westerns countries. This study provides useful insights for understanding the role of the H. pylori dupA gene in disease development. |
| Databáze: | OpenAIRE |
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