Induction and decline of HPRT mutants and deletions following a low dose radiation exposure at Chernobyl
| Autor: | Irene M. Jones, Pavel Pleshanov, David O. Nelson, Cynthia B. Thomas |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Adult
Hypoxanthine Phosphoribosyltransferase Health Toxicology and Mutagenesis Mutant Chromosomal translocation Biology Polymerase Chain Reaction Russia law.invention Andrology Exon Germline mutation law Occupational Exposure Genetics Humans Lymphocytes Molecular Biology Polymerase chain reaction X chromosome Dose-Response Relationship Radiation DNA Middle Aged Clone Cells Hypoxanthine-guanine phosphoribosyltransferase Mutation Mutation (genetic algorithm) Radioactive Hazard Release Gene Deletion Microsatellite Repeats Power Plants |
| Zdroj: | Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 499:177-187 |
| ISSN: | 0027-5107 |
| Popis: | This study was conducted to evaluate the ability of mutation in the hypoxanthine-phosphoribosyltransferase gene (HPRT) to detect radiation-induced mutation in lymphocytes of Russian Chernobyl Clean-up workers, particularly as a function of time after exposure. It is part of a multi-endpoint study comparing HPRT mutation with chromosome translocation and glycophorin A mutation [Radiat. Res. 148 (1997) 463], and extends an earlier report on HPRT [Mutat. Res. 431 (1999) 233] by including data from all 9 years of our study (versus the first 6 years) and analysis of deletion size. Blood samples were collected from 1991 to 1999. HPRT mutant frequency (MF) as determined by the cloning assay was elevated 16% in Clean-up workers (N=300, the entire group minus one outlier) compared to Russian Controls (N=124) when adjusted for age and smoking status (P=0.028). Since exposures occurred over a short relative to the long sampling period, the year of sampling corresponded roughly to the length of time since exposure (correlation coefficient=0.94). When date of blood sample was considered, Control MF was not time dependent. Clean-up worker MF was estimated to be 47% higher than Control MF in 1991 (P=0.004) and to decline 4.4% per year thereafter (P=0.03). A total of 1123 Control mutants and 2799 Clean-up worker mutants were analyzed for deletion type and size by PCR assay for retention of HPRT exons and flanking markers on the X chromosome. There was little difference between the overall deletion spectra of Clean-up workers and Controls. However, there was a decline in the average size of deletions of Clean-up workers as time after exposure at Chernobyl increased from 6 to 13 years (P< or =0.05). The results illustrate the sensitivity of HPRT somatic mutation as a biomarker for populations with low dose radiation exposure, and the dependence of this sensitivity on time elapsed since radiation exposure. |
| Databáze: | OpenAIRE |
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