MALDI-Imaging Mass Spectrometry on Tissues
| Autor: | Athanasios Gotsopoulos, Veronica Mainini, Vasiliki Bitsika, Marc Baumann, Maciej Lalowski, Fulvio Magni |
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| Přispěvatelé: | Antonia Vlahou, Manousos Mkridakis, Mainini, V, Lalowski, M, Gotsopoulos, A, Bitsika, V, Baumann, M, Magni, F |
| Rok vydání: | 2014 |
| Předmět: |
MALDI imaging
Analyte BIO/12 - BIOCHIMICA CLINICA E BIOLOGIA MOLECOLARE CLINICA Computational biology Mass spectrometry Proteomics 01 natural sciences Mass spectrometry imaging Imaging 03 medical and health sciences In patient 030304 developmental biology 0303 health sciences On-tissue digestion Chemistry 010401 analytical chemistry Proteomic spettrometria di massa BIO/10 - BIOCHIMICA Small molecule digestive system diseases 0104 chemical sciences 3. Good health Proteomica Clinical proteomic Sample integrity |
| Zdroj: | Methods in Molecular Biology ISBN: 9781493918713 |
| Popis: | Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF)—profiling and imaging mass spectrometry (MSI) are promising technologies for measuring hundreds of different molecules directly on tissues. For instance, small molecules, drugs and their metabolites, endogenous lipids, carbohydrates and complex peptides/proteins can be measured at the same time. In the most advanced instruments, it is achieved without significant disruption of sample integrity. MSI is a unique approach for assessing the spatial distribution of molecules using graphical multidimensional maps of their constituent analytes, which may for instance be correlated with histopathological alterations in patient tissues. MALDI-TOFMSI technology has been implemented in hospitals of several countries, where it is routinely used for quick pathogen(s) identification, a task formerly accomplished by laborious and expensive DNA/RNA-based PCR (polymerase chain reaction) screening.In this chapter, we describe how MSI is performed, what is required from the researcher, the instrument vendors and finally what can be achieved with MSI. We restrict our descriptions only to MALDI- MSI although several other MS techniques of ionization can easily be linked to MSI. |
| Databáze: | OpenAIRE |
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