Effect of the methodology on circulating peptides determination and consequences on net flux measurements across the gastrointestinal tract of sheep
| Autor: | V. Buchan, Laurence Bernard, Didier Rémond, D. Wilson, C. F. R. Backwell, J.C. MacRae, L. A. Bruce |
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| Přispěvatelé: | Unité de Recherches sur les Herbivores (URH), Institut National de la Recherche Agronomique (INRA) |
| Rok vydání: | 2002 |
| Předmět: |
Male
030309 nutrition & dietetics Ultrafiltration Peptide Intestinal absorption 03 medical and health sciences Hydrolysis chemistry.chemical_compound Animals Perchloric acid Amino Acids ComputingMilieux_MISCELLANEOUS chemistry.chemical_classification 0303 health sciences Sulfosalicylic acid Chromatography Sheep Chemistry Benzenesulfonates 0402 animal and dairy science Reproducibility of Results 04 agricultural and veterinary sciences 040201 dairy & animal science Salicylates Amino acid Molecular Weight Intestinal Absorption Sephadex [SDV.SA.SPA]Life Sciences [q-bio]/Agricultural sciences/Animal production studies Animal Science and Zoology Peptides |
| Zdroj: | Archives of Animal Nutrition Archives of Animal Nutrition, Informa UK (Taylor & Francis), 2002, 56, pp.13-21 HAL |
| ISSN: | 0003-942X 1477-2817 |
| Popis: | Two methodologies for the measurement of peptide amino acids (PAA) in blood were compared to evaluate their effects on the measurement of the net flux of peptides across the gastrointestinal tract of sheep. These methods consisted of a chemical deproteinization of blood samples with sulfosalicylic acid (1.6 M, 0.1 ml for 1 ml of sample) or perchloric acid (1 M, 1 ml for 1 ml of sample) followed by ultrafiltration through a 3,000-Da cut-off filter (SSA + UF3 kD) or gel filtration through a Sephadex G-15 column (1,500-Da cut-off filter; PCA + G-15), respectively, prior to PAA analysis. Peptide concentrations as determined by amino acid concentrations before and after hydrolysis of samples were slightly greater with the SSA + UF3 kD (991 microM) than with the PCA + G-15 (605 microM) methodology. However, both methodologies gave similar net portal-drained viscera flux data in sheep fed on alfalfa pellets with histidine as the only significant uptake of peptide amino acid. |
| Databáze: | OpenAIRE |
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