Construction of new T vectors for direct cloning of PCR products
| Autor: | Yoshikazu Kurosawa, Yoshikazu Ichihara |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Cloning
Base Sequence Genetic Vectors Molecular Sequence Data General Medicine Biology Molecular cloning Polymerase Chain Reaction Molecular biology TA cloning law.invention Restriction enzyme chemistry.chemical_compound chemistry law Mutagenesis Site-Directed Genetics TOPO cloning Amino Acid Sequence Vector (molecular biology) Cloning Molecular Deoxyribonucleases Type II Site-Specific Thymine Polymerase chain reaction Taq polymerase |
| Zdroj: | Gene. 130:153-154 |
| ISSN: | 0378-1119 |
| Popis: | More than half of the products of PCR contain an extra A residue at the 3' end, which is the result of the template-independent activity of Taq polymerase. To facilitate cloning of the products of PCR without modification, T vectors, which have a single overhanging T residue at the 3' end, have been developed. In the present study, we constructed new T vectors which can be prepared in the laboratory by simple digestion with the restriction enzymes AspEI or Eam1 105I. |
| Databáze: | OpenAIRE |
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