Macrophage differentiation of human precursor B-cell line by phorbol ester and colony-stimulating factor
| Autor: | Harvey D. Preisler, Atsunobu Hiraoka, Jun Minowada, Kazuo Kubota |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Naphthol AS D Esterase
Liquid culture Immunology Biology Biochemistry Phorbol ester Cell Line B cell line Colony-Stimulating Factors Phagocytosis Macrophage differentiation Cell Adhesion Round cell Humans B-Lymphocytes Dose-Response Relationship Drug integumentary system Macrophages Cell Differentiation Cell Biology Hematology Colony-stimulating factor Phorbols Molecular biology Tetradecanoylphorbol Acetate Pseudopodia Stem cell line |
| Zdroj: | Blood. 59:997-1000 |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood.v59.5.997.bloodjournal595997 |
| Popis: | A human round cell line, KLM-2, is considered to be precursor B-cell by immunologic surface marker analysis and histochemical studies. The effect of 12-O-tetradecanoyl phorbol-13-acetate (TPA) and/or colony- stimulating factor (CSF) on KLM-2 cells was investigated. KLM-2 cells became adherent to the bottom of the flask on day 2 after liquid culture with TPA, and the number of macrophage-like adherent cells (mAC) with pseudopodia showed a TPA dose-dependent increase to the peak level on day 3 or 4. The mAC had a phagocytic activity. The suspending cells were positive for alpha-naphthyl acetate esterase (NAE) up to about 50% in culture with TPA. A TPA concentration of 100 ng/ml was optimal to provide a maximum number of mAC and NAE-positive suspending cells. Particularly, such effects of TPA on KLM-2 cells were markedly accelerated in the presence of CSF. These findings indicate macrophage differentiation of KLM-2 cells in the presence of TPA and CSF. KLM-2 cells may be a bipotential stem cell line differentiating into B-cell and monocyte-macrophage lineages. |
| Databáze: | OpenAIRE |
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