A Multifunctional Graphene Oxide Platform for Targeting Cancer

Autor: Eva Majkova, Vlasta Zavisova, Martina Kubovcikova, Peter Siffalovic, Zdenko Spitalsky, Mária Omastová, Miroslav Šlouf, Martina Koneracka, Matej Mičušík, Miriam Zaťovičová, Michal Bodík, Nikola Bugarova, Ivana Kajanova, Silvia Pastorekova
Rok vydání: 2019
Předmět:
Zdroj: Cancers
Volume 11
Issue 6
Cancers, Vol 11, Iss 6, p 753 (2019)
ISSN: 2072-6694
DOI: 10.3390/cancers11060753
Popis: Diagnosis of oncological diseases remains at the forefront of current medical research. Carbonic Anhydrase IX (CA IX) is a cell surface hypoxia-inducible enzyme functionally involved in adaptation to acidosis that is expressed in aggressive tumors
hence, it can be used as a tumor biomarker. Herein, we propose a nanoscale graphene oxide (GO) platform functionalized with magnetic nanoparticles and a monoclonal antibody specific to the CA IX marker. The GO platforms were prepared by a modified Hummers and Offeman method from exfoliated graphite after several centrifugation and ultrasonication cycles. The magnetic nanoparticles were prepared by a chemical precipitation method and subsequently modified. Basic characterization of GO, such as the degree of oxidation, nanoparticle size and exfoliation, were determined by physical and chemical analysis, including X-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM), energy dispersive X-ray analysis (EDX), and atomic force microscopy (AFM). In addition, the size and properties of the poly-L-lysine-modified magnetic nanoparticles were characterized. The antibody specific to CA IX was linked via an amidic bond to the poly-L-lysine modified magnetic nanoparticles, which were conjugated to GO platform again via an amidic bond. The prepared GO-based platform with magnetic nanoparticles combined with a biosensing antibody element was used for a hypoxic cancer cell targeting study based on immunofluorescence.
Databáze: OpenAIRE
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