Functional characterization of a synthetic hydrophilic antifungal peptide derived from the marine snail Cenchritis muricatus
| Autor: | Simoni Campos Dias, Ilka M. Vasconcelos, Ludovico Migliolo, Osmar N. Silva, Annia Alba, Carlos López-Abarrategui, Carolina Rodrigues Costa, Hilda Garay, Anselmo J. Otero-González, Osvaldo Reyes-Acosta, Octavio L. Franco, Maysa Paula da Costa, Maria do Rosário Rodrigues Silva, Jose T.A. Oliveira |
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| Rok vydání: | 2012 |
| Předmět: |
Models
Molecular Antifungal Agents Erythrocytes Cell Survival Surface Properties Sequence analysis Molecular Sequence Data Snails Antimicrobial peptides Molecular modeling Peptide Microbial Sensitivity Tests Snail Biochemistry Protein Structure Secondary Microbiology Antifungal peptide Mice Residue (chemistry) Protein structure Sequence Analysis Protein biology.animal Animals Humans Amino Acid Sequence Peptide sequence Cells Cultured chemistry.chemical_classification Chromatography Reverse-Phase biology Fungi General Medicine Cenchritis muricatus biology.organism_classification Peptide Fragments Synthetic peptide chemistry Hydrophobic and Hydrophilic Interactions |
| Zdroj: | Biochimie. 94:968-974 |
| ISSN: | 0300-9084 |
| Popis: | Antimicrobial peptides have been found in mollusks and other sea animals. In this report, a crude extract of the marine snail Cenchritis muricatus was evaluated against human pathogens responsible for multiple deleterious effects and diseases. A peptide of 1485.26 Da was purified by reversed-phase HPLC and functionally characterized. This trypsinized peptide was sequenced by MS/MS technology, and a sequence (SRSELIVHQR), named Cm-p1 was recovered, chemically synthesized and functionally characterized. This peptide demonstrated the capacity to prevent the development of yeasts and filamentous fungi. Otherwise, Cm-p1 displayed no toxic effects against mammalian cells. Molecular modeling analyses showed that this peptide possible forms a single hydrophilic α-helix and the probable cationic residue involved in antifungal activity action is proposed. The data reported here demonstrate the importance of sea animals peptide discovery for biotechnological tools development that could be useful in solving human health and agribusiness problems. |
| Databáze: | OpenAIRE |
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