Role Of Glucose Repression In The Oxidative Stress Response Of Schizosaccharomyces Pombe: Analysis Of Transcript Levels Of Fbp1, Hxk2, Sod1 And Ctt1 Genes In Sty1, Atf1 And Pap1 Knock-Out Mutants
| Autor: | Farinaz Jafari Ghods, Bedia Palabiyik, Semian Karaer Uzuner |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Physiology Mutant SOD1 Biology medicine.disease_cause Microbiology Superoxide dismutase 03 medical and health sciences chemistry.chemical_compound Genetics medicine Oxidative stress response glucose repression Atf1 Pap1 Sty1 knock-out deletion Schizosacharomyces pombe Molecular Biology Transcription factor Hexokinase Cell Biology biology.organism_classification Cell biology 030104 developmental biology chemistry Schizosaccharomyces pombe biology.protein Signal transduction General Agricultural and Biological Sciences Oxidative stress Biyoloji |
| Zdroj: | Volume: 40, Issue: 4 815-825 Turkish Journal of Biology |
| ISSN: | 1300-0152 1303-6092 |
| Popis: | Previously we reported that the glucose sensing/signaling pathway affected the oxidative stress response of Schizosaccharomyces pombe, based on studies of glucose-repression and oxidative-stress-resistant mutants including ird5. sty1, encoding a protein kinase, and aft1 and pap1, encoding transcription factors, are important components of the oxidative stress response in S. pombe. To analyze the relationship between the glucose sensing/signaling pathway and the oxidative stress response, we generated sty1, aft1, and pap1 knock-out mutants in ird5 and wild-type backgrounds. We evaluated the survival rates of the ird5 double mutants (5A, 5P, and 5S) and wild-type single mutants (9A, 9P, and 9S) under mild oxidative stress. In addition, we analyzed the transcript levels of genes related to oxidative stress (sod1, encoding superoxide dismutase; ctt1, encoding catalase) and glucose metabolism (fbp1, encoding fructose-1, 6-bisphosphatase; hxk2, encoding hexokinase). All deletion mutants showed very low survival rates under oxidative stress ( |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|