Structure of the Maize Streak Virus Geminate Particle
| Autor: | Margaret I. Boulton, Jeffrey W. Davies, Lee Faulkner, Norman H. Olson, Mavis Agbandje-McKenna, Timothy S. Baker, Robert McKenna, Wei Zhang |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
0106 biological sciences
Cryo-electron microscopy viruses Molecular Sequence Data cryo-electron microscopy icosahedral capsid Sequence alignment Antiparallel (biochemistry) Zea mays 01 natural sciences Protein Structure Secondary 03 medical and health sciences Capsid Protein structure three-dimensional image reconstruction Virology Plant virus Image Processing Computer-Assisted Maize streak virus Amino Acid Sequence Geminiviridae Structural motif 030304 developmental biology 0303 health sciences biology Cryoelectron Microscopy biology.organism_classification Models Structural maize streak virus ssDNA virus Chemical physics Sequence Alignment 010606 plant biology & botany |
| Zdroj: | Virology. 279:471-477 |
| ISSN: | 0042-6822 |
| DOI: | 10.1006/viro.2000.0739 |
| Popis: | The Geminiviridae is an extensive family of plant viruses responsible for economically devastating diseases in crops worldwide. Geminiviruses package circular, single-stranded DNA (ssDNA) genomes. The characteristic twinned or “geminate” particles, which consist of two joined, incomplete T = 1 icosahedra, are unique among viruses. We have determined the first structure of a geminivirus particle, the Nigerian strain of Maize streak virus (MSV-N), using cryo-electron microscopy and three-dimensional image reconstruction methods. The particle, of dimensions 220 × 380 Å, has an overall 52-point-group symmetry, in which each half particle “head” consists of the coat protein (CP) arranged with quasi-icosahedral symmetry. We have modeled the MSV-N CP as an eight-stranded, antiparallel β-barrel motif (a structural motif common to all known ssDNA viruses) with an N-terminal α-helix. This has produced a model of the geminate particle in which 110 copies of the CP nicely fit into the reconstructed density map. The reconstructed density map and MSV-N pseudo-atomic model demonstrate that the geminate particle has a stable, defined structure. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect