Impact of Preanalytical Factors on Calprotectin Concentration in Stool

Autor: Henrike M Hamer, A H Leontine Mulder, Nanne K de Boer, Femke Crouwel, Patrick F van Rheenen, Marlies Spekle, Marloes Vermeer, Lucie Wagenmakers-Huizinga, Anneke C Muller Kobold
Přispěvatelé: Laboratory Medicine, Gastroenterology and hepatology, Amsterdam Gastroenterology Endocrinology Metabolism, Center for Liver, Digestive and Metabolic Diseases (CLDM), Guided Treatment in Optimal Selected Cancer Patients (GUTS)
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Hamer, H M, Mulder, A H L, de Boer, N K, Crouwel, F, van Rheenen, P F, Spekle, M, Vermeer, M, Wagenmakers-Huizinga, L & Muller Kobold, A C 2022, ' Impact of Preanalytical Factors on Calprotectin Concentration in Stool : A Multiassay Comparison ', the Journal of Applied Laboratory Medicine, vol. 7, no. 6, pp. 1401-1411 . https://doi.org/10.1093/jalm/jfac057
the Journal of Applied Laboratory Medicine, 7(6), 1401-1411. Oxford University Press
Journal of applied laboratory medicine, 7(6):jfac057. Oxford University Press
ISSN: 2576-9456
DOI: 10.1093/jalm/jfac057
Popis: Background Measuring calprotectin concentration in stool is increasingly important in monitoring disease activity and treatment response in inflammatory bowel disease. This study evaluates the impact of preanalytical storage conditions on reliability of calprotectin testing using 5 different calprotectin immunoassays. Methods Aliquots of homogenized fresh fecal samples in untreated or extracted form were stored at room temperature or 4°C. Calprotectin concentration was measured day 0 to 4 and 8. Five different immunoassays and accompanying extraction buffers were used (CALiaGold, Phadia EliA, Bühlmann fCal turbo, ELISA Bühlmann, Inova Quanta Flash). Repeated measurements of change from baseline calprotectin levels over time were analyzed using a mixed model analysis. Results Calprotectin concentrations declined over time under all preanalytical conditions with all assays, except for extracted feces stored at 4°C. The rate of decline was greatest in untreated stool kept at room temperature, reaching significant difference from baseline already after 1 day (P < 0.001). In extracted feces kept at room temperature, significant difference from baseline was reached after 2 days, and in untreated feces at 4°C, after 4 days. However, the results differed significantly between assays. After 4 days of storage at room temperature, the mean calprotectin decline from baseline differed between 30% and 60%, dependent on the assay used. Conclusions Fecal calprotectin concentration in stool samples declines over time, and the rate of decline is greater at higher temperatures. In extracted feces stored at 4°C, calprotectin is most stable. It is assay-dependent how long extracted feces stored at 4°C give reliable test results.
Databáze: OpenAIRE
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