Antibody fragments may be incorrectly processed in the yeast Pichia pastoris
| Autor: | T. A. Yagudin, D. G. Kozlov |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Saccharomyces cerevisiae
Molecular Sequence Data Bioengineering Immunoglobulin light chain Applied Microbiology and Biotechnology Antibody fragments Pichia Pichia pastoris Mice Animals Humans Amino Acid Sequence Protein Precursors Immunoglobulin Fragments Ascomycota biology General Medicine biology.organism_classification Yeast Biochemistry Yield (chemistry) biology.protein Electrophoresis Polyacrylamide Gel Antibody Protein Processing Post-Translational Biotechnology |
| Zdroj: | Biotechnology letters. 30(9) |
| ISSN: | 0141-5492 |
| Popis: | We have studied the efficiency of N-terminal processing of the antibody light chain depending on the structure of the leader sequence when expressed in the yeast Pichia pastoris. The humanized light kappa-chain of the murine antibody H3-1 and the Saccharomyces cerevisiae alpha-factor pre-pro-leader sequence (pre-pro-alpha-F) were used as models. The use of pre-region of the pre-pro-alpha-F alone or together with the Glu-Ala-linker leads to the slightly increased yield of the secreted L-chain but was accompanied by the incomplete N-terminal processing of the secreted product. |
| Databáze: | OpenAIRE |
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