Effects induced by bothropstoxin, a component from Bothrops jararacussu snake venom, on mouse and chick muscle preparations
| Autor: | J. Prado-Franceschi, M.I. Homsi-Brandeburgo, JoséR. Giglio, N.F. Heluany, Léa Rodrigues-Simioni |
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| Rok vydání: | 1992 |
| Předmět: |
Male
medicine.medical_specialty Action Potentials Tubocurarine Venom Stimulation Tetrodotoxin Biology In Vitro Techniques Toxicology chemistry.chemical_compound Mice Internal medicine Crotalid Venoms medicine Animals Muscles Depolarization Molecular biology Electric Stimulation Respiratory Muscles Compound muscle action potential Phrenic Nerve EGTA Endocrinology chemistry Snake venom Calcium medicine.symptom Chickens Muscle contraction Muscle Contraction |
| Zdroj: | Toxicon : official journal of the International Society on Toxinology. 30(10) |
| ISSN: | 0041-0101 |
| Popis: | Bothropstoxin, a 13,700 mol. wt myotoxic phospholipase homologue isolated from the venom of Bothrops jararacussu and devoid of PLA2, proteolytic or hemolytic activities, inhibited muscle twitch tension, evoked either directly or indirectly through stimulation of the motor nerve in the mouse phrenic-diaphragm preparations. The compound action potential of the muscle was also abolished with a similar time course. In addition, the toxin (0.7 mM) evoked membrane depolarization which was inhibited in the presence of 10 mM Ca2+. In chick biventer cervicis muscle, the toxin (2 mM) induced a contracture that reached its maximum amplitude in 44.8 +/- 15.6 min (n = 6) and was not blocked by either d-tubocurarine or tetrodotoxin. The time to maximum amplitude was reduced to 5.5 +/- 1.0 min (n = 4) in nominally Ca(2+)-free Krebs solution and was completely abolished in Ca(2+)-free Krebs solution containing 1 mM EGTA. |
| Databáze: | OpenAIRE |
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