Challenges and Opportunities in the Development of Aptamers for TNFα
| Autor: | Michaela Mai, Bettina Appel, Ingeborg Hospach, Claudia Nübel, Nadejda Krasteva, Gabriele Nelles, Sabine Müller, Lothar Petruschka |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Aptamer Bioengineering Biosensing Techniques Biology 010402 general chemistry 01 natural sciences Applied Microbiology and Biotechnology Biochemistry 03 medical and health sciences RNA Aptamers Humans Surface plasmon resonance Molecular Biology Tumor Necrosis Factor-alpha Rational design General Medicine Quartz crystal microbalance Aptamers Nucleotide Surface Plasmon Resonance In vitro 0104 chemical sciences 030104 developmental biology Quartz Crystal Microbalance Techniques Tumor necrosis factor alpha Biotechnology |
| Zdroj: | Applied Biochemistry and Biotechnology. 179:398-414 |
| ISSN: | 1559-0291 0273-2289 |
| Popis: | RNA aptamers for tumor necrosis factor-alpha (TNFα), for which functionality was demonstrated in L929 cells, show only little affinity for the protein in vitro. Detailed investigation of the aptamer-protein interaction by surface plasmon resonance and quartz crystal microbalance analysis revealed that affinity is not the only crucial parameter for efficacy and functionality of those aptamers. Instead, the sensitive equilibrium of the monomeric and homotrimeric form of soluble TNFα decides on aptamer binding. Our results show that the field of application and the source of TNFα have to be carefully defined before selection of aptamer sequences. |
| Databáze: | OpenAIRE |
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