Generation of human inner ear prosensory-like cells via epithelial-to-mesenchymal transition
| Autor: | Lei Zhang, Hui Jiang, Fengping Dong, Zhengqing Hu, Xuemei Luo, Edwin M. Monsell, Fengqing Lu |
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| Rok vydání: | 2012 |
| Předmět: |
Embryology
Pathology medicine.medical_specialty Epithelial-Mesenchymal Transition Sensory Receptor Cells Biomedical Engineering Cell Separation Biology Article Mesoderm medicine Humans Inner ear Epithelial–mesenchymal transition Saccule and Utricle Progenitor cell Cell Shape Cells Cultured Cell Proliferation Stem Cells Mesenchymal stem cell Epithelial Cells Cell biology Endothelial stem cell Intercellular Junctions medicine.anatomical_structure Ear Inner sense organs Hair cell Stem cell Biomarkers Adult stem cell |
| Zdroj: | Regenerative Medicine. 7:663-673 |
| ISSN: | 1746-076X 1746-0751 |
| Popis: | Aim: To identify human hair cell progenitors from adult inner ear sensory epithelium. Materials & methods: We collected discarded utricles from translabyrinthine surgery and isolated human utricular sensory epithelial cells (HUCs) to explore whether they can proliferate and obtain features of stem/progenitor cells in vitro using reverse transcription PCR and immunofluorescence. Results: When cultured in vitro, HUCs expressed genes and proteins that are usually present in prosensory cells and stem cells. Additionally, dissociated HUCs expanded on the substrates and presented properties of mesenchymal cells via epithelial-to-mesenchymal transition. Conclusion: The results reveal that sensory epithelial cells from the adult human inner ear can re-enter the cell cycle and adopt a stem/progenitor cell fate. The outcomes of this study may open avenues for human hair cell progenitor generation, which could potentially provide a novel stem cell-based replacement for hearing loss and other inner ear disorders. |
| Databáze: | OpenAIRE |
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