Purification method for the isolation of monophosphate nucleotides from Champagne wine and their identification by mass spectrometry
| Autor: | Jerome Aussenac, Bruno Duteurtre, Monique Charpentier, David Chassagne, Catherine Claparols, Claudine Charpentier, Michel Feuillat |
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| Rok vydání: | 2001 |
| Předmět: |
Wine
chemistry.chemical_classification Electrospray Chromatography Nucleotides Chemistry Electrospray ionization Organic Chemistry General Medicine Reversed-phase chromatography Mass spectrometry Biochemistry Lees High-performance liquid chromatography Mass Spectrometry Analytical Chemistry Nucleotide Chromatography High Pressure Liquid |
| Zdroj: | Journal of Chromatography A. 907:155-164 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/s0021-9673(00)01019-0 |
| Popis: | Monophosphate nucleotides are difficult to identify in Champagne wine because they are present in small concentrations in a complex mixture. A method for the isolation, separation and identification of reference compounds, which achieved on average 79% recovery (except for cytidine derivatives), was developed and applied to wine. Some monophosphate nucleotides were then isolated from a Champagne wine aged on lees for 8 years, by ultrafiltration followed by a semi-preparative HPLC step using a strong anion-exchange column. The fraction obtained was subjected to HPLC in a reversed-phase column to remove the salt previously introduced, before identification of compounds by HPLC coupled to a mass spectrometer. For the first time in wine, 5'-IMP, 5'-AMP, 5'-CMP, 5'-GMP, 5'-UMP and the 3'- and/or 2'-isomers of the four latter compounds were identified by comparing their HPLC and electrospray ionization mass spectrometry data with those of reference nucleotides. |
| Databáze: | OpenAIRE |
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