Early Postoperative Endoscopic Recurrence in Crohn’s Disease Is Characterised by Distinct Microbiota Recolonisation

Autor: André D'Hoore, Kathleen Machiels, Albert Wolthuis, João Sabino, Zixuan Xie, Severine Vermeire, Alba Santiago, Gert De Hertogh, Marc Ferrante, Chaysavanh Manichanh, Marta Pozuelo del Río, Victòria Pascal Andreu, Adrian Martinez-De la Torre, David Campos
Rok vydání: 2020
Předmět:
Zdroj: Journal of Crohn's & Colitis
ISSN: 1876-4479
1873-9946
DOI: 10.1093/ecco-jcc/jjaa081
Popis: Background and Aims Intestinal microbiota dysbiosis is implicated in Crohn’s disease [CD] and may play an important role in triggering postoperative disease recurrence [POR]. We prospectively studied faecal and mucosal microbial recolonisation following ileocaecal resection to identify the predictive value of recurrence-related microbiota. Methods Mucosal and/or faecal samples from 121 CD patients undergoing ileocaecal resection were collected at predefined time points before and after surgery. Ileal biopsies were collected from 39 healthy controls. POR was defined by a Rutgeerts score ≥i2b. The microbiota was evaluated by 16S rRNA sequencing. Prediction analysis was performed using C5.0 and Random Forest algorithms. Results The mucosa-associated microbiota in CD patients was characterised by a depletion of butyrate-producing species (false discovery rate [FDR] Conclusions Microbiota recolonisation after ileocaecal resection is different between recurrence and non-recurrence patients, with Fusobacteria as the most prominent player driving early POR. These bacteria involved in the early recolonisation and POR represent a promising therapeutic strategy in the prevention of disease recurrence.
Databáze: OpenAIRE
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