The effect of interleukin-17 on the proliferation and invasion of JEG-3 human choriocarcinoma cells
| Autor: | Donruedee Sanguansermsri, Puntharee Butkhamchot, Kwansuda Supalap, Sutatip Pongcharoen, Pannika R. Niumsup |
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| Rok vydání: | 2006 |
| Předmět: |
medicine.medical_specialty
Receptor expression Immunology Interleukin-17 receptor Biology Multinucleate Internal medicine Cell Line Tumor medicine Immunology and Allergy Humans Decidual cells Neoplasm Invasiveness Choriocarcinoma RNA Messenger Receptor Cell Proliferation Receptors Interleukin-17 Cell growth Interleukin-17 Obstetrics and Gynecology Trophoblast Receptors Interleukin Immunohistochemistry Cell biology Trophoblasts Endocrinology medicine.anatomical_structure Reproductive Medicine Cell culture embryonic structures |
| Zdroj: | American journal of reproductive immunology (New York, N.Y. : 1989). 55(4) |
| ISSN: | 1046-7408 |
| Popis: | Problem As there has been a study in mice showing the expression of IL-17 by decidual cells and the status of IL-17 receptor expression in human pregnancy is not known, we hypothesized that IL-17 may regulate human trophoblast proliferation and invasion. Method of study JEG-3 cell line was used as a model for human trophoblast. Immunohistochemitry and reverse transcriptase polymerase chain reaction techniques were used to identify IL-17 receptor protein and mRNA, respectively. The effects of IL-17 on JEG-3 cell proliferation and invasion were tested using the BrdU incorporation and the Matrigel invasion assays, respectively. Results IL-17 increased the invasive capacity of JEG-3 cells but had no effect on the proliferation and multinucleated formation of JEG-3 cells. Conclusion In this JEG-3 cell model of human trophoblast, the IL-17R and IL-17 may have a regulatory role in trophoblast invasion. |
| Databáze: | OpenAIRE |
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