Androgen Biosynthesis in Leydig Cells after Testicular Desensitization by Luteinizing Hormone-Releasing ormone and Human Chorionic Gonadotropin
| Autor: | M L, Dufau, S, Cigorraga, A J, Baukal, S, Sorrell, J M, Bator, J F, Neubauer, K J, Catt |
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| Rok vydání: | 1979 |
| Předmět: |
Male
endocrine system medicine.medical_specialty medicine.drug_class Receptors Cell Surface Gonadotropin-releasing hormone In Vitro Techniques Chorionic Gonadotropin Human chorionic gonadotropin Gonadotropin-Releasing Hormone Andrology chemistry.chemical_compound Endocrinology Internal medicine medicine Animals Receptor Androgen biosynthetic process Chemistry Leydig Cells Luteinizing Hormone Androgen Rats Kinetics Androgens Spironolactone Luteinizing hormone hormones hormone substitutes and hormone antagonists Hormone |
| Zdroj: | Endocrinology. 105:1314-1321 |
| ISSN: | 1945-7170 0013-7227 |
| DOI: | 10.1210/endo-105-6-1314 |
| Popis: | LHRH-induced elevation of endogenous LH in adult male rats was followed by dose-dependent loss of testicular LH receptors and cAMP responses to hCG stimulation in vitro. Leydig cells from animals treated with 10-100 jtxg LHRH to induce over 50% receptor loss showed reduced testosterone responses to hCG, dibutyryl cAMP, and choleragen. However, prognenolone formation (measured in the presence of 17β-hydroxy- 4,4,17-trimethyl-3-oxoandrost-5-ene-2a-carbonitrile and spironolactone) was normal or increased after LHRH treatment. When LHRH-desensitized cells were stimulated with hCG in vitro, the marked decrease in testosterone production was accompanied by accumulation of 17a-hydroxyprogesterone, either alone or with progesterone. These findings localized the major steroidogenic block in LHRH-desensitized cells at the site of conversion of the 17a-hydroxylated steroid to androgen, similar to the lesion observed in Leydig cells from animals treated with iv hCG. In the latter, the steroidogenic defects were more ... |
| Databáze: | OpenAIRE |
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