Construction of a high-density SNP-based genetic map and identification of fruit-related QTLs and candidate genes in peach [Prunus persica (L.) Batsch]
Autor: | Jicheng Zheng, Caiping Zhao, Pei Shi, Xiaofei Ma, Dong Zhang, Libo Xing, Xianju Wang, Ze Xu, Juanjuan Ma, Shaoyu Zhang, Mingyu Han |
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Rok vydání: | 2020 |
Předmět: |
0106 biological sciences
0301 basic medicine Candidate gene Genetic Linkage QTL Quantitative Trait Loci Single-nucleotide polymorphism Locus (genetics) Plant Science Quantitative trait locus Biology Genes Plant Candidate Gene Identification Polymorphism Single Nucleotide 01 natural sciences 03 medical and health sciences Quantitative Trait Heritable Gene mapping Genetic linkage lcsh:Botany Fruit quality-related traits SNP-based map Prunus persica Genetics Chromosome Mapping food and beverages Peach lcsh:QK1-989 030104 developmental biology Fruit Fruit tree Research Article SLAF 010606 plant biology & botany |
Zdroj: | BMC Plant Biology BMC Plant Biology, Vol 20, Iss 1, Pp 1-16 (2020) |
ISSN: | 1471-2229 |
Popis: | Background High-density genetic mapping is a valuable tool for mapping loci that control specific traits for perennial fruit trees. Peach is an economically important fruit tree and a model Rosaceae species for genomic and genetic research. In peach, even though many molecular markers, genetic maps and QTL mappings have been reported, further research on the improvement of marker numbers, map densities, QTL accuracy and candidate gene identification is still warranted. Results A high-density single nucleotide polymorphism (SNP)-based peach linkage map was constructed using specific locus amplified fragment sequencing (SLAF-seq). This genetic map consisted of 7998 SLAF markers, spanning 1098.79 cM with an average distance of 0.17 cM between adjacent markers. A total of 40 QTLs and 885 annotated candidate genes were detected for 10 fruit-related traits, including fruit weight (FW), fruit diameter (FD), percentage of red skin colour (PSC), eating quality (EQ), fruit flavour (FV), red in flesh (RF), red around pit (RP), adherence to pit (AP), fruit development period (FDP) and fruit fibre content (FFC). Eighteen QTLs for soluble solid content (SSC) were identified along LGs 1, 4, 5, and 6 in 2015 and 2016, and 540 genes were annotated in QTL intervals. Thirty-two QTLs for fruit acidity content (FA) were detected on LG1, and 2, 4, 5, 6, and 1232 candidate genes were identified. The expression profiles of 2 candidate genes for SSC and 4 for FA were analysed in parents and their offspring. Conclusions We constructed a high-density genetic map in peach based on SLAF-seq, which may contribute to the identification of important agronomic trait loci. Ninety QTLs for 12 fruit-related traits were identified, most of which overlapped with previous reports, and some new QTLs were obtained. A large number of candidate genes for fruit-related traits were screened and identified. These results may improve our understanding of the genetic control of fruit quality traits and provide useful information in marker-assisted selection for fruit quality in peach breeding programmes. |
Databáze: | OpenAIRE |
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