Allicin functionalized locust bean gum nanoparticles for improved therapeutic efficacy: An in silico, in vitro and in vivo approach
| Autor: | Annie Abraham, R.S. Soumya, Sainulabdeen Sherin, Kozhiparambil Gopalan Raghu |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cell Survival Phytochemicals Nanoparticle Apoptosis 02 engineering and technology Galactans Biochemistry Mannans Mice 03 medical and health sciences chemistry.chemical_compound Drug Delivery Systems Liver Function Tests Structural Biology In vivo Plant Gums Animals MTT assay Disulfides Molecular Biology Drug Carriers Allicin Chemistry Biological activity General Medicine Sulfinic Acids 021001 nanoscience & nanotechnology In vitro Rats Drug Liberation RAW 264.7 Cells 030104 developmental biology Toxicity Nanoparticles Locust bean gum 0210 nano-technology Biomarkers |
| Zdroj: | International Journal of Biological Macromolecules. 109:740-747 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2017.11.065 |
| Popis: | The field of nanotechnology has overgrown over the past few years and has even ventured into the field of medicine. The aim of the present study is to develop a novel allicin functionalized locust bean gum nanoparticle using the nanoprecipitation technique. The synthesized nanoparticles were characterized by dynamic light scattering, scanning electron microscopy and transmission electron microscopy. The characterization study revealed the nanoscale structure (∼100nm) of the prepared particles. In silico toxicology analysis were carried out to assess the drug-like properties and virtual toxicity of allicin. Toxicity of the prepared nanoparticles were carried out in RAW 264.7 cell lines in vitro and in vivo studies were carried out in Sprague-Dawley rats. In in vitro study, LBGAN showed a maximum toxicity of 10.51% in MTT assay, no reactive oxygen species generation on DCFDA staining and LBGAN was effective to protect the cells from apoptosis. In in vivo toxicity studies LBGAN showed no significant change in the activities of the marker enzymes like LDH, CK-MB, ALP, ACP, AST and ALT. Thus, the functionalization of nanoparticles with allicin has the benefit of providing protection and stability to the allicin, in addition to increasing its pharmacological activity. |
| Databáze: | OpenAIRE |
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