Detection of human herpesvirus 6 (HHV-6) and human herpesvirus 7 (HHV-7) DNA in endocervical samples from a positive and negative HPV woman of Córdoba, Argentina
Autor: | Jorge Pavan, Patricia Biganzoli, Ana Ximena Kiguen, Jessica Paola Mosmann, Fernando Venezuela, María Celia Frutos, Cecilia Cuffini, Leonardo J. Ferreyra |
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Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
Adult Adolescent viruses Herpesvirus 6 Human Argentina Roseolovirus Infections Uterine Cervical Neoplasms Herpesvirus 7 Human Virus Pathology and Forensic Medicine Human Papillomavirus DNA Tests 03 medical and health sciences chemistry.chemical_compound Young Adult 0302 clinical medicine Predictive Value of Tests Atypia medicine Humans Human papillomavirus Cervix Papillomaviridae Retrospective Studies biology business.industry Papillomavirus Infections virus diseases General Medicine biochemical phenomena metabolism and nutrition Middle Aged medicine.disease biology.organism_classification Prognosis Uterine Cervical Dysplasia Molecular biology Human herpesvirus 7 HHV-7 030104 developmental biology medicine.anatomical_structure Uterine cervix chemistry Molecular Diagnostic Techniques 030220 oncology & carcinogenesis DNA Viral Human herpesvirus 6 Female Squamous Intraepithelial Lesions of the Cervix business DNA |
Zdroj: | Journal of clinical pathology. 73(1) |
ISSN: | 1472-4146 |
Popis: | AimsThe purpose of the present study was to elucidate the presence of human herpesvirus 6A (HHV-6A), HHV-6B and HHV-7 in samples of the uterine cervix through detection of viral DNA. We analysed normal tissues, samples with low-grade squamous intraepithelial lesions (LSILs) and high-grade squamous intraepithelial lesions (HSILs). We correlated the presence of HHV-6 and HHV-7 with the finding of human papillomavirus (HPV) in mucosal samples.MethodsCervical samples were examined and grouped as follows: group 1 (n=29), normal cytology; group 2 (n=61), samples with LSIL; group 3 (n=35), samples with HSIL. Molecular biology examinations were performed in all samples to detect HHV-6, HHV-7 and HPV DNA and to typify HHV-6 species.ResultsGroup 1: normal cytology and HPV (−): HHV-6: 6.8% (2/29), HHV-7: 79.3% (23/29); group 2: LSIL and HPV (−): HHV-6: 93.1% (27/29), HHV-7: 96.5% (28/29); LSIL and HPV (+): HHV-6: 0% (0/32), HHV-7: 90.6% (29/32); group 3: HSIL and HPV (−): HHV-6: 20% (2/10), HHV-7: 70% (7/10); HSIL HPV (+): HHV-6: 12% (3/25), HHV-7: 68% (17/25). HHV-6A DNA was not detected in any samples.Conclusions(1) Both HHV-6 and HHV-7 infect the mucosal cells of the cervix with higher prevalence of HHV-7. (2) The higher prevalence of HHV-6 in LSIL HPV (−) samples compared with those with normal cytology indicates that it constitutes a possible risk factor for atypia production. (3) The presence of HHV-7 in all samples questions its role in the production of atypia. (4) The finding of HHV-6 and HHV-7 suggests that the cervical mucosa is a possible transmission pathway for these viruses. |
Databáze: | OpenAIRE |
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