Detection of human herpesvirus 6 (HHV-6) and human herpesvirus 7 (HHV-7) DNA in endocervical samples from a positive and negative HPV woman of Córdoba, Argentina

Autor: Jorge Pavan, Patricia Biganzoli, Ana Ximena Kiguen, Jessica Paola Mosmann, Fernando Venezuela, María Celia Frutos, Cecilia Cuffini, Leonardo J. Ferreyra
Rok vydání: 2019
Předmět:
0301 basic medicine
Adult
Adolescent
viruses
Herpesvirus 6
Human

Argentina
Roseolovirus Infections
Uterine Cervical Neoplasms
Herpesvirus 7
Human

Virus
Pathology and Forensic Medicine
Human Papillomavirus DNA Tests
03 medical and health sciences
chemistry.chemical_compound
Young Adult
0302 clinical medicine
Predictive Value of Tests
Atypia
medicine
Humans
Human papillomavirus
Cervix
Papillomaviridae
Retrospective Studies
biology
business.industry
Papillomavirus Infections
virus diseases
General Medicine
biochemical phenomena
metabolism
and nutrition

Middle Aged
medicine.disease
biology.organism_classification
Prognosis
Uterine Cervical Dysplasia
Molecular biology
Human herpesvirus 7 HHV-7
030104 developmental biology
medicine.anatomical_structure
Uterine cervix
chemistry
Molecular Diagnostic Techniques
030220 oncology & carcinogenesis
DNA
Viral

Human herpesvirus 6
Female
Squamous Intraepithelial Lesions of the Cervix
business
DNA
Zdroj: Journal of clinical pathology. 73(1)
ISSN: 1472-4146
Popis: AimsThe purpose of the present study was to elucidate the presence of human herpesvirus 6A (HHV-6A), HHV-6B and HHV-7 in samples of the uterine cervix through detection of viral DNA. We analysed normal tissues, samples with low-grade squamous intraepithelial lesions (LSILs) and high-grade squamous intraepithelial lesions (HSILs). We correlated the presence of HHV-6 and HHV-7 with the finding of human papillomavirus (HPV) in mucosal samples.MethodsCervical samples were examined and grouped as follows: group 1 (n=29), normal cytology; group 2 (n=61), samples with LSIL; group 3 (n=35), samples with HSIL. Molecular biology examinations were performed in all samples to detect HHV-6, HHV-7 and HPV DNA and to typify HHV-6 species.ResultsGroup 1: normal cytology and HPV (−): HHV-6: 6.8% (2/29), HHV-7: 79.3% (23/29); group 2: LSIL and HPV (−): HHV-6: 93.1% (27/29), HHV-7: 96.5% (28/29); LSIL and HPV (+): HHV-6: 0% (0/32), HHV-7: 90.6% (29/32); group 3: HSIL and HPV (−): HHV-6: 20% (2/10), HHV-7: 70% (7/10); HSIL HPV (+): HHV-6: 12% (3/25), HHV-7: 68% (17/25). HHV-6A DNA was not detected in any samples.Conclusions(1) Both HHV-6 and HHV-7 infect the mucosal cells of the cervix with higher prevalence of HHV-7. (2) The higher prevalence of HHV-6 in LSIL HPV (−) samples compared with those with normal cytology indicates that it constitutes a possible risk factor for atypia production. (3) The presence of HHV-7 in all samples questions its role in the production of atypia. (4) The finding of HHV-6 and HHV-7 suggests that the cervical mucosa is a possible transmission pathway for these viruses.
Databáze: OpenAIRE
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