A robust lithographic method for multiplex surface pattering
| Autor: | J. M. de la Fuente, Huabing Yin, Mathis O. Riehle, Carlos Cuestas-Ayllon, Jonathan M. Cooper, Q. Xiao, Andrew Glidle |
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| Předmět: |
Chemistry
0206 medical engineering Mannose Nanotechnology Sequence (biology) 02 engineering and technology Surface engineering 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences 020601 biomedical engineering 0104 chemical sciences Analytical Chemistry Extracellular matrix chemistry.chemical_compound Silanization Click chemistry Molecule 0210 nano-technology Cell adhesion |
| Zdroj: | Scopus-Elsevier Current Analytical Chemistry ResearcherID |
| Popis: | Surface engineering provides a powerful tool to create model systems to investigate the complex interactions between cells and the extracellular matrix (ECM). Cell adhesive peptide ligands (e.g. Arginine-Glycine-Aspatate (RGD) sequence) and carbohydrates are abundant in the ECM, and both are involved in mediating cell adhesion. However, how these interact is not well understood in vivo. Model systems to investigate their collective roles are rare. We have developed a robust surface patterning method allowing spatial control of carbohydrate (i.e. mannose) and RGD on glass while overcoming non-specific biomolecular interactions. The method effectively combines a traditional photolithographic process with two robust chemical pathways, namely click chemistry and silanization, in order to tailor the surface chemistry. This method is especially promising for the quantitative immobilization of different molecules and holds potential for the investigation of interactions between carbohydrates and common ECM proteins on cell adhesion. - See more at: http://www.eurekaselect.com/105477/article#sthash.LKEggb6P.dpuf |
| Databáze: | OpenAIRE |
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