Early rise in inflammation and microcirculatory disorder determine the development of autoimmune pancreatitis in the MRL/Mp-mouse
| Autor: | Brit Fitzner, Saleh M. Ibrahim, Heiko Sorg, Stephanie-Anna Holzhueter, Robert Jaster, Brigitte Vollmar, Horst Nizze, Benjamin Lorch |
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| Rok vydání: | 2008 |
| Předmět: |
CD4-Positive T-Lymphocytes
Male Pathology medicine.medical_specialty Mice Inbred MRL lpr Pancreatic disease Physiology Inflammation Biology CD8-Positive T-Lymphocytes medicine.disease_cause Autoimmunity Autoimmune Diseases Mice Physiology (medical) Pancreatic cancer Pancreatitis Chronic medicine Leukocytes Animals Pancreas Autoimmune pancreatitis Hepatology Microcirculation Gastroenterology medicine.disease Immunohistochemistry Pathophysiology Disease Models Animal Antibodies Antinuclear Immunology Etiology Pancreatitis Female medicine.symptom |
| Zdroj: | American journal of physiology. Gastrointestinal and liver physiology. 295(6) |
| ISSN: | 0193-1857 |
| Popis: | Autoimmune pancreatitis (AIP) is a rare cause of chronic pancreatitis and mimics pancreatic cancer. Although there is strong interest in research, etiology and pathophysiology of AIP are still unknown. Therefore, we analyzed a total of 92 MRL/Mp-mice of either sex, which are prone to develop AIP, in four different age groups (8–12, 16–20, 24–28, and 32–40 wk). Using intravital fluorescence microscopy, histology, laboratory analysis, and Western blot, onset, severity, and pathophysiological mechanisms of AIP were evaluated. Female animals showed in vivo an age-dependent increase of intrapancreatic leukocyte accumulation, as well as a loss in functional capillary perfusion. In contrast, intrapancreatic inflammation in male mice was less pronounced and not age dependent. Furthermore, pancreatic tissue specimen of female animals exhibited major organ destruction with significantly higher values of mean pathological scores (1.5 ± 0.3 vs. ≤0.2; P < 0.05), as well as significantly increased CD4-, CD8-, CD11b-, and CD138-positive cells compared with male animals of the same age. Interestingly, there was a significant positive correlation between intravascular leukocyte adherence and the histopathological score of the pancreas, indicating a determining role of the innate immune system for the late onset of AIP. The present study shows that the onset of AIP is characterized by an inflammatory response and microcirculatory failure, most probably constituting initiators and propagators of this autoimmune disease. |
| Databáze: | OpenAIRE |
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