Epigenetics in type 1 diabetes: TNFa gene promoter methylation status in Chilean patients with type 1 diabetes mellitus
| Autor: | Ethel Codner, Diego F. Garcia-Diaz, Francisco Pérez-Bravo, Viviana Arroyo-Jousse |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adult Male medicine.medical_specialty Pediatric Obesity Homocysteine Adolescent Medicine (miscellaneous) Nucleic Acid Denaturation Body Mass Index Epigenesis Genetic 03 medical and health sciences chemistry.chemical_compound Young Adult Folic Acid Internal medicine medicine Humans Epigenetics Chile Child Promoter Regions Genetic Whole blood Type 1 diabetes Nutrition and Dietetics business.industry Tumor Necrosis Factor-alpha Promoter Methylation DNA Methylation medicine.disease 030104 developmental biology Endocrinology Diabetes Mellitus Type 1 chemistry Case-Control Studies Cancer research Female business TCF7L2 Biomarkers Epigenetics of diabetes Type 2 |
| Zdroj: | The British journal of nutrition. 116(11) |
| ISSN: | 1475-2662 |
| Popis: | TNF-αis a pro-inflammatory cytokine that is involved in type 1 diabetes (T1D) pathogenesis. TheTNFagene is subject of epigenetic regulation in which folate and homocysteine are important molecules because they participate in the methionine cycle where the most important methyl group donor (S-adenosylmethionine) is formed. We investigated whetherTNFagene promoter methylation status in T1D patients was related to blood folate, homocysteine and TNF-αin a transversal case–control study. We studied T1D patients (n25, mean=13·7 years) and healthy control subjects (n25, mean=31·1 years), without T1D and/or other autoimmune diseases or direct family history of these diseases. A blood sample was obtained for determination of serum folate, plasma homocysteine and TNF-αconcentrations. Whole blood was used for the extraction of DNA to determine the percentage of methylation by real-time PCR and melting-curve analysis. Results are expressed as means and standard deviations for parametric variables and as median (interquartile range) for non-parametric variables. T1D patients showed a higherTNFagene promoter methylation (39·2 (sd19·5) %) when compared with control subjects (25·4 (sd13·7) %) (P=0·008).TNFagene promoter methylation was positively associated only with homocysteine levels in T1D patients (r0·55,P=0·007), but not in control subjects (r−0·122,P=0·872). To our knowledge, this is the first work that reports the methylation status of theTNFagene promoter and its relationship with homocysteine metabolism in Chilean T1D patients without disease complications. |
| Databáze: | OpenAIRE |
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