Regulation of Human β-Cell Adhesion, Motility, and Insulin Secretion by Collagen IV and Its Receptor α1β1
| Autor: | Vincenzo Cirulli, Mayra Yebra, Thomas Kaido, Anthony M.P. Montgomery |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Collagen Type IV
Integrins medicine.medical_specialty Time Factors Cell Survival Integrin Morphogenesis Motility Cell Separation Biochemistry Integrin alpha1beta1 Collagen receptor Islets of Langerhans Cell Movement Internal medicine Insulin Secretion Cell Adhesion medicine Humans Insulin Glucose homeostasis Cell adhesion Receptor Pancreas Molecular Biology Cells Cultured Aged geography geography.geographical_feature_category biology Cell Biology Middle Aged Flow Cytometry Islet Immunohistochemistry Glucose Endocrinology Microscopy Fluorescence biology.protein Collagen |
| Zdroj: | Journal of Biological Chemistry. 279:53762-53769 |
| ISSN: | 0021-9258 |
| Popis: | Collagens have been shown to influence the survival and function of cultured beta-cells; however, the utilization and function of individual collagen receptors in beta-cells is largely unknown. The integrin superfamily contains up to five collagen receptors, but we have determined that alpha(1)beta(1) is the primary receptor utilized by both fetal and adult beta-cells. Cultured beta-cells adhered to and migrated on collagen type IV (Col-IV), and these responses were mediated almost exclusively by alpha(1)beta(1). The migration of cultured beta-cells to Col-IV significantly exceeded that to other matrix components suggesting that this substrate is of unique importance for beta-cell motility. The interaction of alpha(1)beta(1) with Col-IV also resulted in significant insulin secretion at basal glucose concentrations. A subset of beta-cells in developing islets was confirmed to express alpha(1)beta(1), and this expression co-localized with Col-IV in the basal membranes of juxtaposed endothelial cells. Our findings indicate that alpha(1)beta(1) and Col-IV contribute to beta-cell functions known to be important for islet morphogenesis and glucose homeostasis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|