High-fat diet induces systemic B-cell repertoire changes associated with insulin resistance
| Autor: | Khoa Dinh Nguyen, Melissa Hui Yen Chng, Scott D. Boyd, J-Y Lee, Tho D. Pham, Jacob Glanville, Edgar G. Engleman, Krishna M. Roskin, Katherine J. L. Jackson |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Immunoglobulin A medicine.medical_specialty Intra-Abdominal Fat Immunology Receptors Antigen B-Cell Adipose tissue Inflammation Biology Diet High-Fat Mice 03 medical and health sciences 0302 clinical medicine Germline mutation Insulin resistance Antigen Cell Movement Internal medicine medicine Animals Immunology and Allergy Obesity Cells Cultured B-Lymphocytes High-Throughput Nucleotide Sequencing medicine.disease Complementarity Determining Regions Mice Inbred C57BL 030104 developmental biology Endocrinology biology.protein Somatic Hypermutation Immunoglobulin Insulin Resistance Antibody medicine.symptom Transcriptome 030215 immunology |
| Zdroj: | Mucosal Immunology. 10:1468-1479 |
| ISSN: | 1933-0219 |
| Popis: | The development of obesity-associated insulin resistance is associated with B-lymphocyte accumulation in visceral adipose tissue (VAT) and is prevented by B-cell ablation. To characterize potentially pathogenic B-cell repertoires in this disorder, we performed high-throughput immunoglobulin (Ig) sequencing from multiple tissues of mice fed high-fat diet (HFD) and regular diet (RD). HFD significantly changed the biochemical properties of Ig heavy-chain complementarity-determining region-3 (CDRH3) sequences, selecting for IgA antibodies with shorter and more hydrophobic CDRH3 in multiple tissues. A set of convergent antibodies of highly similar sequences found in the VAT of HFD mice but not RD mice showed significant somatic mutation, suggesting a response shared between mice to a common antigen or antigens. These findings indicate that a simple high-fat dietary intervention has a major impact on mouse B-cell repertoires, particularly in adipose tissues. |
| Databáze: | OpenAIRE |
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