Comprehensive Mapping of Long-Range Interactions Reveals Folding Principles of the Human Genome
| Autor: | Maxim Imakaev, Ido Amit, Peter J. Sabo, Job Dekker, Agnes Telling, Michael O. Dorschner, Nynke L. van Berkum, Louise Williams, Leonid A. Mirny, Mark Groudine, Erez Lieberman Aiden, John A. Stamatoyannopoulos, Andreas Gnirke, Richard Sandstrom, Tobias Ragoczy, Bryan R. Lajoie, Eric S. Lander, Bradley E. Bernstein, Michaël Bender |
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| Rok vydání: | 2009 |
| Předmět: |
Models
Molecular Chromatin Immunoprecipitation Protein Conformation Biotin Locus (genetics) Computational biology Biology Genome Article Chromosome conformation capture Higher Order Chromatin Structure Chromosomes Human Humans In Situ Hybridization Fluorescence Cell Line Transformed Gene Library Genomic organization Chromatin Fiber Cell Nucleus Genetics Principal Component Analysis Multidisciplinary Genome Human Computational Biology DNA Sequence Analysis DNA Chromatin Nucleic Acid Conformation Human genome Chromatin Loop Monte Carlo Method |
| Zdroj: | Science. 326:289-293 |
| ISSN: | 1095-9203 0036-8075 |
| DOI: | 10.1126/science.1181369 |
| Popis: | Chromosomal Mapping The conformation of the genome in the nucleus and contacts between both proximal and distal loci influence gene expression. In order to map genomic contacts, Lieberman-Aiden et al. (p. 289 , see the cover) developed a technique to allow the detection of all interactions between genomic loci in the eukaryotic nucleus followed by deep sequencing. This technology was used to map the organization of the human genome and to examine the spatial proximity of chromosomal loci at one megabase resolution. The map suggests that the genome is partitioned into two spatial compartments that are related to local chromatin state and whose remodeling correlates with changes in the chromatin state. |
| Databáze: | OpenAIRE |
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