Restoration of Spermatogenesis and Fertility in Azoospermic Mutant Mice by Suppression and Reelevation of Testosterone Followed by Intracytoplasmic Sperm Injection
| Autor: | Kayoko Dohmae, Akihiko Okuyama, Tsuyoshi Okuno, Yoshitake Nishimune, Kiyomi Matsumiya, Akira Tohda, Masaru Okabe, Hidefumi Kishikawa |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Male
Infertility Cytoplasm endocrine system medicine.medical_specialty medicine.medical_treatment Reproductive technology Biology Intracytoplasmic sperm injection Male infertility Gonadotropin-Releasing Hormone Mice Hormone Antagonists Internal medicine medicine Animals Testosterone Sperm Injections Intracytoplasmic Spermatogenesis In vitro fertilisation urogenital system Cell Differentiation Dipeptides Oligospermia Cell Biology General Medicine medicine.disease Spermatids Spermatozoa Sperm Spermatogonia Mice Inbred C57BL Meiosis Fertility Endocrinology Reproductive Medicine Mutation Oocytes Female |
| Zdroj: | Biology of Reproduction. 66:85-90 |
| ISSN: | 1529-7268 0006-3363 |
| Popis: | Advances in assisted reproduction techniques such as in vitro fertilization and intracytoplasmic sperm injection have made paternity possible for many patients with male infertility. However, at least some sperm or spermatids are required for these techniques to be successful, and patients incapable of producing spermatids cannot be helped. Male mice homozygous for the mutant juvenile spermatogonial depletion (jsd) gene show spermatogonial arrest and an elevated intratesticular testosterone level like many other experimental infertility models such as those with iradiation- or chemotherapy-induced testicular damage. In this category of infertile males, suppression of the testosterone level induces spermatogonial differentiation to the stage of spermatocytes but no further. In the present study with jsd mutant mice, we induced spermatogenesis first to spermatocytes and then to elongated spermatids by suppression of testosterone levels with a GnRH antagonist, Nal-Glu, at a dose of 2500 microg kg(-1) day(-1) for 4 wk and then withdrawal of Nal-Glu. Spermatids were seen in the cross-sections of seminiferous tubules in all mice treated by administration and subsequent withdrawal of Nal-Glu. Four weeks after withdrawal of Nal-Glu, some of the germ cells differentiated into elongated spermatids. Supplementation with testosterone and Nal-Glu after 4 wk of treatment with Nal-Glu alone also induced spermatogenesis similar to the induction by withdrawal of Nal-Glu. Thus, we ascribe the restoration of the differentiation of spermatocytes to spermatids to reelevation of the testosterone level. Furthermore, we successfully rescued male sterility in jsd mice by subsequent intracytoplasmic sperm injection using the elongated spermatids induced by the programmed hormone therapy. |
| Databáze: | OpenAIRE |
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