Laminectomy and Spinal Cord Window Implantation in the Mouse
| Autor: | Sarah E. Lutz, Simon Alford, Elizabeth A. Pietruczyk, Terilyn K. L. Stephen |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Materials science
General Immunology and Microbiology Heartbeat General Chemical Engineering General Neuroscience medicine.medical_treatment Laminectomy Mice Transgenic Prostheses and Implants Spinal cord General Biochemistry Genetics and Molecular Biology Mice medicine.anatomical_structure Spinal Cord Two-photon excitation microscopy Isoflurane Microscopy medicine Fluorescence microscope Animals Plastics Preclinical imaging Biomedical engineering medicine.drug |
| Zdroj: | Journal of Visualized Experiments. |
| ISSN: | 1940-087X |
| DOI: | 10.3791/58330 |
| Popis: | This protocol describes a method for spinal cord laminectomy and glass window implantation for in vivo imaging of the mouse spinal cord. An integrated digital vaporizer is utilized to achieve a stable plane of anesthesia at a low-flow rate of isoflurane. A single vertebral spine is removed, and a commercially available cover-glass is overlaid on a thin agarose bed. A 3D-printed plastic backplate is then affixed to the adjacent vertebral spines using tissue adhesive and dental cement. A stabilization platform is used to reduce motion artifact from respiration and heartbeat. This rapid and clamp-free method is well-suited for acute multi-photon fluorescence microscopy. Representative data are included for an application of this technique to two-photon microscopy of the spinal cord vasculature in transgenic mice expressing eGFP:Claudin-5 - a tight junction protein. |
| Databáze: | OpenAIRE |
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