Radical scavenging-linked anti-adipogenic activity of Alnus firma extracts
| Autor: | Hee-Sun Yang, Sarah Lee, Jong Seok Lee, Sun-Il Choi, Il-Jun Kang, Boo-Yong Lee, Jong Yea Kim, Joo-Hong Yeo, Ok-Hwan Lee, Jin-Ha Lee |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Antioxidant Oxygen radical absorbance capacity medicine.medical_treatment Flavonoid Biology Alnus Fatty Acid-Binding Proteins Nitric Oxide Antioxidants Alnus firma Anthocyanins 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine anti-oxidant assay 3T3-L1 Cells Genetics medicine Animals Food science Gallic acid 3T3-L1 chemistry.chemical_classification Reactive oxygen species Adipogenesis Plant Extracts Gene Expression Regulation Developmental Catechin General Medicine Articles Free Radical Scavengers Lipid Metabolism PPAR gamma 030104 developmental biology chemistry CCAAT-Binding Factor 030220 oncology & carcinogenesis total phenolic content Cancer research Quercetin Reactive Oxygen Species anti-adipogenic |
| Zdroj: | International Journal of Molecular Medicine |
| ISSN: | 1791-244X 1107-3756 |
| Popis: | The purpose of the present study was to investigate the antioxidant activity and anti-adipogenic effect of extracts from Alnus firma (A. firma), which is an edible plant that grows in mountainous areas. The total phenolic, flavonoid and anthocyanin content as well as the antioxidant activity of a 70% ethanolic extract of A. firma (AFE) was assessed. Furthermore, the effects of AFE on lipid accumulation and reactive oxygen species (ROS) production during adipogenesis of 3T3-L1 cells were investigated. The results revealed that the total phenolic, flavonoid and pro-anthocyanidin content of AFE as 436.26±3.30 mg gallic acid equivalents/g, 73.82±0.54 mg quercetin equivalents/g and 149.25±6.06 mg catechin equivalents/g, respectively. In addition, AFE exerted significant antioxidant effects in terms of 1,1-diphenyl-2-picryl hydrazyl radical scavenging activity, 2,2′-azino-bis(3-ethylbenzothia-zoline-6-sulfonic acid) radical scavenging activity, reducing power, oxygen radical absorbance capacity and nitric oxide radical scavenging activity. As for its anti-adipogenic activity, AFE significantly inhibited ROS production and lipid accumulation during adipogenesis of 3T3-L1 cells compared with those in control cells. In addition, AFE regulated adipogenic transcription factors including peroxisome proliferator-activated receptor-γ, CCAAT/enhance-binding protein α and adipocyte protein 2. These results indicated that A. firma is a potential candidate for a functional food supplement. |
| Databáze: | OpenAIRE |
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