A collagen-binding protein on the surface of ejaculated rabbit spermatozoa
| Autor: | E D Nudelman, J K Koehler, S Hakomori |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1980 |
| Předmět: |
Male
endocrine system Fluorescent Antibody Technique Biology Chromatography Affinity Affinity chromatography Capacitation medicine Animals Trypsin Polyacrylamide gel electrophoresis Sperm motility reproductive and urinary physiology Glycoproteins urogenital system Cell Biology Articles Epididymis Sperm Molecular biology Spermatozoa Fibronectins Fibronectin Molecular Weight medicine.anatomical_structure biology.protein Sperm Motility Collagen Rabbits Carrier Proteins medicine.drug |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| Popis: | A rabbit antibody to mouse 3T3 cell fibronectin was used in conjunction with a fluorescein-tagged second antibody to detect fibronectin-like activity on the surface of rabbit spermatozoa. Only ejaculated sperm displayed an intense and highly localized fluorescence over the acrosomal region. Cauda epididymal sperm of the rabbit as well as several other species did not exhibit any reaction. The fluorescent activity could be eliminated by trypsin treatment but was re-established by incubation in cell-free seminal fluid. Sperm recovered from females 10-12 h after mating showed a reduction or absence of antifibronectin fluorescence, suggesting that this component's loss could be a factor in sperm capacitation. Because fibronectins show strong binding to collagen, mixtures of ejaculated sperm and collagen were examined in the light and electron microscope. Living sperm appear to have a strong affinity for collagen and quickly adhere to the filaments by their heads, while continuing vigorous flagellations. Surface labeling of sperm with the galactose-oxidase-NaB[3H]4 technique, extraction with urea-detergent mixtures and affinity chromatography of extracts on gelatin-Sepharose revealed a single radioactive band of mot wt approximately 40,000 after SDS polyacrylamide gel electrophoresis and fluorography. |
| Databáze: | OpenAIRE |
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