Molecular Lipid Films on Microengineering Materials
Autor: | Samuel Lara-Avila, Tatsiana Lobovkina, Irep Gözen, Siegfried Eigler, Alar Ainla, Karolina Spustova, Severin Schindler, Silver Jõemetsa, Aldo Jesorka, Kiryl Kustanovich |
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Rok vydání: | 2019 |
Předmět: |
Materials science
Phospholipid 02 engineering and technology Surface engineering 010402 general chemistry 01 natural sciences law.invention chemistry.chemical_compound law Electrochemistry General Materials Science Lipid bilayer Spectroscopy Graphene Borosilicate glass Surfaces and Interfaces Adhesion 021001 nanoscience & nanotechnology Condensed Matter Physics 0104 chemical sciences Membrane chemistry Chemical engineering lipids (amino acids peptides and proteins) Mica 0210 nano-technology |
Zdroj: | Langmuir : the ACS journal of surfaces and colloids. 35(32) |
ISSN: | 1520-5827 |
Popis: | In this study, we have systematically investigated the formation of molecular phospholipid films on a variety of solid substrates fabricated from typical surface engineering materials and the fluidic properties of the lipid membranes formed on these substrates. The surface materials comprise of borosilicate glass, mica, SiO2, Al (native oxide), Al2O3, TiO2, ITO, SiC, Au, Teflon AF, SU-8, and graphene. We deposited the lipid films from small unilamellar vesicles (SUVs) by means of an open-space microfluidic device, observed the formation and development of the films by laser scanning confocal microscopy, and evaluated the mode and degree of coverage, fluidity, and integrity. In addition to previously established mechanisms of lipid membrane–surface interaction upon bulk addition of SUVs on solid supports, we observed nontrivial lipid adhesion phenomena, including reverse rolling of spreading bilayers, spontaneous nucleation and growth of multilamellar vesicles, and the formation of intact circular patches of double lipid bilayer membranes. Our findings allow for accurate prediction of membrane–surface interactions in microfabricated devices and experimental environments where model membranes are used as functional biomimetic coatings. |
Databáze: | OpenAIRE |
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