Epidermal growth factor promotes rapid response to epithelial injury in rabbit duodenum in vitro
| Autor: | Enrico P. Cosentini, Bela Teleky, Feil W, Martin Riegler, Rudolf Schiessel, Etienne Wenzl, Georg Bischof, Tacettin Sogukoglu, Gerhard Hamilton, Roland Sedivy |
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| Rok vydání: | 1996 |
| Předmět: |
medicine.medical_specialty
Duodenum medicine.medical_treatment In Vitro Techniques Epithelium Cell Movement Epidermal growth factor Internal medicine medicine Animals Insulin-Like Growth Factor I Intestinal Mucosa Lagomorpha Epidermal Growth Factor Hepatology biology Ussing chamber Growth factor Gastroenterology biology.organism_classification Molecular biology In vitro Electrophysiology medicine.anatomical_structure Endocrinology Cytokine Rabbits |
| Zdroj: | Gastroenterology. 111:28-36 |
| ISSN: | 0016-5085 |
| DOI: | 10.1053/gast.1996.v111.pm8698221 |
| Popis: | Growth factors are mainly involved in the regulation of intestinal epithelial barrier function. This study investigated the effect of epidermal growth factor (EGF) and insulin-like growth factor 1 (IGF-1) on epithelial restitution of rabbit duodenum in vitro.Rabbit duodenal mucosal strips mounted in an Ussing chamber were luminally exposed to 10 mmol/L HCl for 10 minutes and then incubated with buffer alone or luminal buffer containing various concentrations of EGF and IGF-1 for 3 hours. Resistance was calculated from potential difference and short-circuit current. Damage was assessed by morphometry on HE-stained sections.HCl caused resistance to decrease from 112 +/- 2 to 51 +/- 4 ohms x cm2 10 minutes after injury (n = 6; P0.05). Postinjury treatment with 25 or 50 ng/mL luminal EGF for 3 hours stimulated resistance to recover to 94 +/- 3 and 104 +/- 3 ohms x cm2, respectively, vs. 81 +/- 3 omega x cm2 in controls (P0.05). Ten minutes after injury, 62% of the mucosa was damaged; 3 hours after injury, damage was reduced to 24% +/- 1.09% and 10% +/- 1.42% in the 25 and 50 ng/mL EGF group, respectively, vs. 38% +/- 0.93% in controls (n = 6 per group). EGF stimulated enterocyte migration. IGF-1 did not impair epithelial restitution.EGF, but not IGF-1, promoted epithelial restitution of rabbit duodenum in vitro. |
| Databáze: | OpenAIRE |
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