DNA polymerase epsilon is required for heterochromatin maintenance in Arabidopsis

Autor: Ángeles Gómez-Zambrano, David Latrasse, Steven E. Jacobsen, Thierry Pélissier, Amy Hesketh, Leticia López-González, Moussa Benhamed, Pierre Bourguet, Magdalena E. Potok, Olivier Mathieu, Magali Perez, Marie-Noëlle Pouch-Pélissier, Olivier Da Ines, Charles I. White
Přispěvatelé: Génétique, Reproduction et Développement (GReD ), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), Instituto de Bioquimica Vegetal y Fotosintesis (IBVF), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Department of Molecular, Cell and Developmental Biology [Los Angeles], University of California [Los Angeles] (UCLA), University of California (UC)-University of California (UC), Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), CNRS, Inserm, Universite Clermont Auvergne Region Auvergne-Rhone-Alpes European Molecular Biology Organization (EMBO) European Research Council (ERC)European CommissionI2ST 260742Ministere de l'education nationale, de l'enseignement superieur et de la recherche United States Department of Health & Human ServicesNational Institutes of Health (NIH) - USAR35 GM130272, Instituto de Bioquimica Vegetal y Fotosintesis, University of California-University of California, Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Howard Hughes Medical Institute (HHMI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Génétique, Reproduction et Développement (GReD), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA), University of California, White, Charles
Jazyk: angličtina
Rok vydání: 2020
Předmět:
DNA Replication
0106 biological sciences
[SDV.BIO]Life Sciences [q-bio]/Biotechnology
Euchromatin
Heterochromatin
Arabidopsis
DNA polymerase epsilon
[SDV.CAN]Life Sciences [q-bio]/Cancer
[SDV.GEN] Life Sciences [q-bio]/Genetics
Biology
[SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry
Molecular Biology/Molecular biology
01 natural sciences
Epigenesis
Genetic
Histones
[SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics
03 medical and health sciences
[SDV.CAN] Life Sciences [q-bio]/Cancer
[SDV.GEN.GPL] Life Sciences [q-bio]/Genetics/Plants genetics
Gene Silencing
Epigenetics
Heterochromatin maintenance
030304 developmental biology
0303 health sciences
[SDV.GEN]Life Sciences [q-bio]/Genetics
DNA methylation
Silencing
Arabidopsis Proteins
Research
Cell Cycle
DNA replication
Replication stress
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Molecular biology
DNA Polymerase II
Chromatin
Up-Regulation
[SDV.BIO] Life Sciences [q-bio]/Biotechnology
Cell biology
[SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding
[SDV.BV.AP] Life Sciences [q-bio]/Vegetal Biology/Plant breeding
010606 plant biology & botany
Zdroj: Genome Biology
Genome Biology, 2020, 21 (1), ⟨10.1186/s13059-020-02190-1⟩
Genome Biology, BioMed Central, 2020, 21 (1), ⟨10.1186/s13059-020-02190-1⟩
Genome Biology, BioMed Central, 2020, 21, ⟨10.1186/s13059-020-02190-1⟩
Digital.CSIC. Repositorio Institucional del CSIC
instname
ISSN: 1465-6906
1474-760X
DOI: 10.1186/s13059-020-02190-1⟩
Popis: Background Chromatin organizes DNA and regulates its transcriptional activity through epigenetic modifications. Heterochromatic regions of the genome are generally transcriptionally silent, while euchromatin is more prone to transcription. During DNA replication, both genetic information and chromatin modifications must be faithfully passed on to daughter strands. There is evidence that DNA polymerases play a role in transcriptional silencing, but the extent of their contribution and how it relates to heterochromatin maintenance is unclear. Results We isolate a strong hypomorphic Arabidopsis thaliana mutant of the POL2A catalytic subunit of DNA polymerase epsilon and show that POL2A is required to stabilize heterochromatin silencing genome-wide, likely by preventing replicative stress. We reveal that POL2A inhibits DNA methylation and histone H3 lysine 9 methylation. Hence, the release of heterochromatin silencing in POL2A-deficient mutants paradoxically occurs in a chromatin context of increased levels of these two repressive epigenetic marks. At the nuclear level, the POL2A defect is associated with fragmentation of heterochromatin. Conclusion These results indicate that POL2A is critical to heterochromatin structure and function, and that unhindered replisome progression is required for the faithful propagation of DNA methylation throughout the cell cycle.
Databáze: OpenAIRE
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