The Effect of Endogenous Adenosine on Neuronal Activity in Rats: An FDG PET Study
| Autor: | Ji Hyun Ko, Fiona E. Parkinson, Soumen Paul, Shadreck Mzengeza, Dali Zhang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Adenosine medicine.medical_treatment Short Communication Morpholines Intraperitoneal injection Short Communications Endogeny Adenosine kinase 03 medical and health sciences Adenosine A1 receptor 0302 clinical medicine Fluorodeoxyglucose F18 Internal medicine medicine Image Processing Computer-Assisted Premovement neuronal activity Animals Humans Radiology Nuclear Medicine and imaging Brain Mapping biology business.industry Receptor Adenosine A1 adenosine A1 receptor Antagonist Brain Glucose analog 3. Good health Rats 030104 developmental biology Endocrinology Glucose Pyrimidines Positron-Emission Tomography Xanthines biology.protein Neurology (clinical) in vivo imaging business Glycolysis 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Journal of Neuroimaging |
| ISSN: | 1552-6569 1051-2284 |
| Popis: | 2–18F‐fluorodeoxy‐D‐glucose (FDG) is a glucose analog that is taken up by cells and phosphorylated. The amount of FDG accumulated by cells is a measure of the rate of glycolysis, which reflects cellular activity. As the levels and actions of the neuromodulator adenosine are dynamically regulated by neuronal activity, this study was designed to test whether endogenous adenosine affects tissue accumulation of FDG as assessed by positron emission tomography (PET) or by postmortem analysis of tissue radioactivity. Rats were given an intraperitoneal injection of the adenosine A1 receptor antagonist 8‐cyclopentyl‐1,3‐dipropyl‐xanthine (DPCPX, 3 mg/kg), the adenosine kinase inhibitor ABT‐702 (3 mg/kg), or vehicle 10 minutes prior to an intravenous injection of FDG (15.4 ± 0.7 MBq per rat). Rats were then subjected to a 15 minute static PET scan. Reconstructed images were normalized to FDG PET template for rats and standard uptake values (SUVs) were calculated. To examine the regional effect of active treatment compared to vehicle, statistical parametric mapping analysis was performed. Whole‐brain FDG uptake was not affected by drug treatment. Significant regional hypometabolism was detected, particularly in cerebellum, of DPCPX‐ and ABT‐702 treated rats, relative to vehicle‐treated rats. Thus, endogenous adenosine can affect FDG accumulation although this effect is modest in quiescent rats. |
| Databáze: | OpenAIRE |
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