Identification of a 374 amino acid protein encoded by RNA segment 6 of influenza C virus
Autor: | Kiyoto Nakamura, Fumio Kitame, Yasushi Muraki, Kanetsu Sugawara, P Gao, Yuichiro Tada, Seiji Hongo, Yoko Matsuzaki |
---|---|
Rok vydání: | 1998 |
Předmět: |
endocrine system
Glycosylation Influenzavirus C Chick Embryo Biology Cross Reactions environment and public health Peptide Mapping Polymerase Chain Reaction Cell Line Viral Matrix Proteins Open Reading Frames Viral Proteins Virology Animals Humans RNA Messenger Integral membrane protein Peptide sequence DNA Primers chemistry.chemical_classification Messenger RNA Viral matrix protein Base Sequence RNA Antibodies Monoclonal Molecular biology Amino acid Molecular Weight enzymes and coenzymes (carbohydrates) Biochemistry chemistry COS Cells RNA Viral biological phenomena cell phenomena and immunity Influenza C Virus EF-Tu |
Zdroj: | The Journal of general virology. 79 |
ISSN: | 0022-1317 |
Popis: | Unspliced mRNA from RNA segment 6 of influenza C virus contains a single open reading frame that potentially encodes a polypeptide of 374 amino acids. This polypeptide, which has not been identified as yet, is predicted to contain the complete amino acid sequence of the matrix protein, M1, as well as that of a small integral membrane protein, CM2. Here, we found that small amounts of two previously unrecognized proteins with apparent molecular masses of 42 (P42) and 44 kDa (P44) were immunoprecipitated with immune serum against CM2. The electrophoretic mobilities of P42 and P44 varied depending on virus strain, indicating that they are virus-coded. Treatment of infected cells with tunicamycin and digestion of immunoprecipitated proteins with various endoglycosidases revealed that P42 is modified by the addition of a high-mannose oligosaccharide chain to generate P44. A monoclonal antibody against M1, like anti-CM2 serum, was able to immunoprecipitate both the P42 and P44 proteins. Furthermore, the tryptic peptide map of either P42 or P44 was indistinguishable from the map of the mixture of M1 and CM2. These results, taken together, suggest strongly that P42 and P44 correspond to the 374 amino acid protein encoded by unspliced RNA segment 6 mRNA and its N-glycosylated form, respectively. |
Databáze: | OpenAIRE |
Externí odkaz: |