Enhanced shRNA delivery by the combination of polyethylenimine, ultrasound, and nanobubbles in liver cancer
| Autor: | Dongsheng Zhang, Hongbo Li, Chenyan Yuan, Hao Zhang, Jia Zhang, Ziyu Wang, Xinxin Hou |
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| Rok vydání: | 2019 |
| Předmět: |
Carcinoma
Hepatocellular Genetic enhancement Survivin 0206 medical engineering Biomedical Engineering Biophysics Health Informatics Bioengineering Apoptosis 02 engineering and technology Gene delivery Biomaterials Small hairpin RNA 03 medical and health sciences 0302 clinical medicine shRNA Medicine Gene silencing Humans Polyethyleneimine Molecular Targeted Therapy RNA Small Interfering Ultrasonography Microbubbles business.industry ultrasound Liver Neoplasms Gene Transfer Techniques Cancer medicine.disease 020601 biomedical engineering transfection gene carrier Nanobubbles Cancer cell embryonic structures Cancer research business Liver cancer 030217 neurology & neurosurgery Information Systems Research Article |
| Zdroj: | Technology and Health Care |
| ISSN: | 1878-7401 |
| Popis: | Background Traditional cancer treatments such as surgery, radiation, and chemotherapy destroy both cancer and normal cells, which limit their clinical application. It is difficult to achieve the best results for any liver cancer patients using any single treatment method. Gene therapy for HCC demands non-invasive, efficient, targeted and safe gene transfection strategies. Objective In this study, a nonviral shRNA gene delivery system utilizing a combination of PEI, US, and NBs was developed for targeting survivin in liver Cancer. Methods and results The PEI-shRNA-NBs cumulated in the tumor tissue because of the EPR effect. By exposure to the US, micelles shRNA may be released from PEI-shRNA-NBs in tumor tissues and the shRNA then transmitted efficiently to cancer cells. Considerably enhanced therapeutic outcome was obtained with the gene silencing effect enhanced. Conclusions PEI-shRNA-NBs possess the potential to become promising tools intended for shRNA delivery. |
| Databáze: | OpenAIRE |
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