Stable isotope labeling methods for DNA
| Autor: | Hans A. Heus, Marco Tessari, Frank H. T. Nelissen, Sybren S. Wijmenga |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Nuclear and High Energy Physics 010402 general chemistry 01 natural sciences Biochemistry Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound Isotopes Molecule Nucleotide Nuclear Magnetic Resonance Biomolecular Spectroscopy chemistry.chemical_classification Binding Sites DNA synthesis Isotope Nucleotides Chemistry Stable isotope ratio DNA 0104 chemical sciences 030104 developmental biology Enzyme Isotope Labeling Nucleic acid Biophysical Chemistry |
| Zdroj: | Progress in Nuclear Magnetic Resonance Spectroscopy, 96, 89-108 Progress in Nuclear Magnetic Resonance Spectroscopy, 96, pp. 89-108 |
| ISSN: | 0079-6565 |
| Popis: | NMR is a powerful method for studying proteins and nucleic acids in solution. The study of nucleic acids by NMR is far more challenging than for proteins, which is mainly due to the limited number of building blocks and unfavorable spectral properties. For NMR studies of DNA molecules, (site specific) isotope enrichment is required to facilitate specific NMR experiments and applications. Here, we provide a comprehensive review of isotope-labeling strategies for obtaining stable isotope labeled DNA as well as specifically stable isotope labeled building blocks required for enzymatic DNA synthesis. |
| Databáze: | OpenAIRE |
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