Systemic Retinoic Acid Treatment Induces Sodium/Iodide Symporter Expression and Radioiodide Uptake in Mouse Breast Cancer Models
| Autor: | Lisa H. Che, Gregory A. Brent, James J. Schultz, Yoko Kanamoto, Katsumi Taki, Farhad Moatamed, Takahiko Kogai |
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| Rok vydání: | 2004 |
| Předmět: |
Sodium-iodide symporter
Genetically modified mouse Cancer Research medicine.medical_specialty Transplantation Heterologous Retinoic acid Breast Neoplasms Tretinoin Mice SCID Biology Iodine Radioisotopes Mice chemistry.chemical_compound Breast cancer Cell Line Tumor Internal medicine medicine Animals Humans Tissue Distribution Radionuclide Imaging skin and connective tissue diseases reproductive and urinary physiology Symporters Oncogene medicine.disease Gene Expression Regulation Neoplastic Transplantation Endocrinology Oncology chemistry embryonic structures Cancer cell Symporter Cancer research Female |
| Zdroj: | Cancer Research. 64:415-422 |
| ISSN: | 1538-7445 0008-5472 |
| DOI: | 10.1158/0008-5472.can-03-2285 |
| Popis: | Lactating breast tissue and some breast cancers express the sodium/iodide symporter (NIS) and concentrate iodide. We recently demonstrated that all-trans retinoic acid (tRA) induces both NIS gene expression and iodide accumulation in vitro in well-differentiated human breast cancer cells (MCF-7). In the present study, we investigated the in vivo efficacy and specificity of tRA-stimulated iodide accumulation in mouse breast cancer models. Immunodeficient mice with MCF-7 xenograft tumors were treated with systemic tRA for 5 days. Iodide accumulation in the xenograft tumors was markedly increased, ∼15-fold greater than levels without treatment, and the effects were tRA dose dependent. Iodide accumulation in other organs was not significantly influenced by tRA treatment. Significant induction of NIS mRNA and protein in the xenograft tumors was observed after tRA treatment. Iodide accumulation and NIS mRNA expression were also selectively induced in breast cancer tissues in transgenic mice expressing the oncogene, polyoma virus middle T antigen. These data demonstrate selective induction of functional NIS in breast cancer by tRA. Treatment with short-term systemic retinoic acid, followed by radioiodide administration, is a potential tool in the diagnosis and treatment of some differentiated breast cancer. |
| Databáze: | OpenAIRE |
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