Transfection of fluorescent probed antisense compounds: L-Cysteine derivatives of nucleic acid bases
| Autor: | S, Kitaoka, Y, Inaki, H, Maruoka, N, Tohnai, T, Hatae, T, Tanabe |
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| Rok vydání: | 2001 |
| Předmět: |
RNase P
Ribonuclease H Transfection Oligodeoxyribonucleotides Antisense chemistry.chemical_compound Nucleic Acids Tumor Cells Cultured Fluorescence microscope Animals Fluorescent Dyes Cell Nucleus biology Chemistry Adenine Carbocysteine RNA General Medicine Proteinase K Fluorescence humanities eye diseases Microscopy Fluorescence Biochemistry embryonic structures Nucleic acid biology.protein Fluorescein Endopeptidase K Peptides human activities Thymine DNA |
| Zdroj: | Nucleic Acids Symposium Series. 1:135-136 |
| ISSN: | 1746-8272 0261-3166 |
| DOI: | 10.1093/nass/1.1.135 |
| Popis: | Antisense with L-cysteine derivative (CAS) can recognize DNA and forms the complementary duplex with DNA. So the properties of CAS in vitro and in vivo were examined in this study. CAS was resistant to proteinase K and stabilized RNA against RNase HI. Moreover using fluorescent CAS, the localization was observed by fluorescence microscope and confocal microscope. As a result, CASs were accumulated inside the nucleus in NG108-15. |
| Databáze: | OpenAIRE |
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