Resolution and Identification of Ribosomes from Mycobacteria
| Autor: | D. W. Smith, E. H. Wiegeshaus, L. Trnka |
|---|---|
| Rok vydání: | 1968 |
| Předmět: |
Tris
Genetics and Molecular Biology medicine.disease_cause Microbiology Ribosome Analytical Ultracentrifugation chemistry.chemical_compound Ribonucleases Bacterial Proteins Polysome Centrifugation Density Gradient Escherichia coli medicine Magnesium Molecular Biology Differential centrifugation Carbon Isotopes biology Nucleotides Ribosomal RNA Mycobacterium bovis chemistry Biochemistry biology.protein Pancreatic ribonuclease Ribosomes Ultracentrifugation |
| Zdroj: | Journal of Bacteriology. 95:310-313 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.95.2.310-313.1968 |
| Popis: | Ribosomes were isolated from mycobacteria (BCG) by differential centrifugation by use of a modification of Nirenberg's procedure. Individual components of the ribosomal spectrum were resolved in linear sucrose density gradients. Sedimentation constants of the individual components were determined by analytical ultracentrifugation. Ribosomes from escherichia coli labeled with 14 C-uracil were used as markers for an independent confirmation of the identity of individual peaks. The typical ribosomal spectrum of 70 S units and 50 S and 30 S subunits was obtained in tris(hydroxymethyl)aminomethane buffer with 0.01 m Mg(CH 3 COO) 2 . This corresponds to the ribosomal spectrum obtained in E. coli under comparable conditions. In 0.0001 m Mg 2+ , the 70 S units were dissociated to 50 S and 30 S particles. Pancreatic ribonuclease produced no significant changes in main ribosomal components. The isolation techniques used in this work precluded the recognition of polyribosomes. |
| Databáze: | OpenAIRE |
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