tom-1, a novel v-Myb target gene expressed in AMV- and E26-transformed myelomonocytic cells
| Autor: | Bénédicte Haenig, Karl-Heinz Klempnauer, Oliver Burk, Susanne Worpenberg |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Gene Expression Regulation
Viral animal structures Genes Viral Molecular Sequence Data Retroviridae Proteins Oncogenic Biology DNA-binding protein General Biochemistry Genetics and Molecular Biology Oncogene Proteins v-myb Animals MYB Amino Acid Sequence Cloning Molecular Binding site Promoter Regions Genetic Molecular Biology Transcription factor Gene Avian Myeloblastosis Virus Avian Leukosis Virus Base Sequence General Immunology and Microbiology Ccaat-enhancer-binding proteins General Neuroscience fungi Nuclear Proteins Promoter Oncogenes Cell Transformation Viral Molecular biology DNA-Binding Proteins Cell Transformation Neoplastic DNA Viral CCAAT-Enhancer-Binding Proteins Chickens Research Article |
| Zdroj: | The EMBO Journal. 16:1371-1380 |
| ISSN: | 1460-2075 |
| DOI: | 10.1093/emboj/16.6.1371 |
| Popis: | The retroviral oncogene v-myb is a mutated and truncated version of the c-myb proto-oncogene and encodes a transcription factor (v-Myb) that specifically transforms myelomonocytic cells. Two different variants of v-myb, transduced independently by the oncogenic chicken retroviruses AMV and E26, have been characterized. It is believed that both variants of v-Myb transform myelomonocytic cells by affecting the expression of specific genes; however, no target genes common to both oncogenic viruses have been identified. Here, we describe the identification of a novel v-Myb target gene, designated as tom-1 (target of myb 1). The tom-1 gene has two promoters, one of which is Myb-inducible. tom-1 is expressed at elevated levels in AMV-transformed as well as in E26-transformed myeloid cells. We show that tom-1 activation by v-Myb does not require de novo protein synthesis and that the Myb-inducible tom-1 promoter contains a functional Myb binding site. Thus, tom-1 is the first example of a direct target gene for both oncogenic forms of the v-myb gene. Further analysis of the Myb-inducible tom-1 promoter shows that a C/EBP binding site is juxtaposed to the Myb binding site and that C/EBP is required for the Myb-dependent activation of the promoter. Together with previous work our results suggest that C/EBP may be a general cooperation partner for v-Myb in myelomonocytic cells. |
| Databáze: | OpenAIRE |
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