Quantitative analysis of hIgG1 in monkey serum by LC–MS/MS using mass spectrometric immunoassay
| Autor: | Christian Lanshoeft, Olivier Kretz, Olivier Heudi, Andrew Paul Warren, Sarah Cianférani, Franck Picard |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Mass spectrometric immunoassay Clinical Biochemistry Tandem mass spectrometry 01 natural sciences Analytical Chemistry Mice 03 medical and health sciences chemistry.chemical_compound Limit of Detection Tandem Mass Spectrometry Trifluoroacetic acid medicine Animals Biotinylation General Pharmacology Toxicology and Pharmaceutics Immunoassay Detection limit Chromatography medicine.diagnostic_test Elution 010401 analytical chemistry General Medicine Intravitreal administration Antibodies Anti-Idiotypic 0104 chemical sciences Macaca fascicularis Medical Laboratory Technology 030104 developmental biology chemistry Immunoglobulin G Quantitative analysis (chemistry) Chromatography Liquid |
| Zdroj: | Bioanalysis. 8:1035-1049 |
| ISSN: | 1757-6199 1757-6180 |
| DOI: | 10.4155/bio.16.32 |
| Popis: | Aim: A sensitive generic LC–MS/MS method for hIgG1 quantification in cynomolgus monkey serum using mass spectrometric immunoassay disposable automation research tips (MSIA-D.A.R.T.'S™) is reported. Results: The hIgG1 was captured with a biotinylated mouse anti-hIgG antibody (50.0 µg/ml) targeting the fragment crystallizable (Fc) region. Elution from the streptavidin-coated MSIA-D.A.R.T.'s was conducted with 0.4% trifluoroacetic acid in water. The method was selective and linear from 10.0 to 1000 ng/ml using 100 µl of serum. The method was evaluated regarding accuracy, precision, carry-over, dilution, auto-sampler stability and applied for the determination of hIgG1 concentration in monkey serum after intravitreal administration. Conclusion: The present assay is suitable for quantitative analysis of hIgG1-based therapeutic proteins in monkey serum at low levels. |
| Databáze: | OpenAIRE |
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